TY  - JOUR
A1  - Stijnis, Kees
A1  - Dijkmans, Anneke C.
A1  - Bart, Aldert
A1  - Brosens, Lodewijk A. A.
A1  - Muntau, Birgit
A1  - Schoen, Christoph
A1  - Barth, Thomas F.
A1  - van Gulik, Thomas
A1  - van Gool, Tom
A1  - Grobusch, Martin P.
A1  - Tappe, Dennis
T1  - Echinococcus vogeli in Immigrant from Suriname to the Netherlands
JF  - Emerging Infectious Diseases
KW  - alveolar echinococcosis
KW  - monoclonal antibody
KW  - multilocularis
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-143953
VL  - 21
IS  - 3
ER  - 
TY  - JOUR
A1  - Barth, Thomas F. E.
A1  - Herrmann, Tobias S.
A1  - Tappe, Dennis
A1  - Stark, Lorenz
A1  - Grüner, Beate
A1  - Buttenschoen, Klaus
A1  - Hillenbrand, Andreas
A1  - Juchems, Markus
A1  - Henne-Bruns, Doris
A1  - Kern, Petra
A1  - Seitz, Hanns M.
A1  - Möller, Peter
A1  - Rausch, Robert L.
A1  - Kern, Peter
A1  - Deplazes, Peter
T1  - Sensitive and Specific Immunohistochemical Diagnosis of Human Alveolar Echinococcosis with the Monoclonal Antibody Em2G11
JF  - PLoS Neglected Tropical Diseases
N2  - Background: Alveolar echinococcosis (AE) is caused by the metacestode stage of Echinococcus multilocularis. Differential diagnosis with cystic echinococcosis (CE) caused by E. granulosus and AE is challenging. We aimed at improving diagnosis of AE on paraffin sections of infected human tissue by immunohistochemical testing of a specific antibody. 
Methodology/Principal Findings: We have analysed 96 paraffin archived specimens, including 6 cutting needle biopsies and 3 fine needle aspirates, from patients with suspected AE or CE with the monoclonal antibody (mAb) Em2G11 specific for the Em2 antigen of E. multilocularis metacestodes. In human tissue, staining with mAb Em2G11 is highly specific for E. multilocularis metacestodes while no staining is detected in CE lesions. In addition, the antibody detects small particles of E. multilocularis (spems) of less than 1 mm outside the main lesion in necrotic tissue, liver sinusoids and lymphatic tissue most probably caused by shedding of parasitic material. The conventional histological diagnosis based on haematoxylin and eosin and PAS stainings were in accordance with the immunohistological diagnosis using mAb Em2G11 in 90 of 96 samples. In 6 samples conventional subtype diagnosis of echinococcosis had to be adjusted when revised by immunohistology with mAb Em2G11. 
Conclusions/Significance: Immunohistochemistry with the mAb Em2G11 is a new, highly specific and sensitive diagnostic tool for AE. The staining of small particles of E. multilocularis (spems) outside the main lesion including immunocompetent tissue, such as lymph nodes, suggests a systemic effect on the host.
KW  - cells
KW  - multilocularis
KW  - antigen
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-135371
VL  - 6
IS  - 10
ER  - 
TY  - JOUR
A1  - Atanasov, Georgi
A1  - Benkert, Christoph
A1  - Thelen, Armin
A1  - Tappe, Dennis
A1  - Frosch, Matthias
A1  - Teichmann, Dieter
A1  - Barth, Thomas F. E.
A1  - Wittekind, Christian
A1  - Schubert, Stefan
A1  - Jonas, Sven
T1  - Alveolar echinococcosis-spreading disease challenging clinicians: A case report and literature review
JF  - World Journal of Gastroenterology
N2  - Human alveolar echinococcosis (AE) is a potentially deadly disease; recent studies have shown that the endemic area of Echinococcus multilocularis, its causative agent, is larger than previously known. This disease has low prevalence and remains underreported in Europe. Emerging clinical data show that diagnostic difficulties are still common. We report on a 76-year old patient suffering from AE lesions restricted to the left lobe of the liver who underwent a curative extended left hemihepatectomy. Prior to the resection a liver biopsy under the suspicion of an atypical malignancy was performed. After the intervention he developed a pseudoaneurysm of the hepatic artery that was successfully coiled. Surprisingly, during surgery, the macroscopic appearance of the tumour revealed a growth pattern that was rather typical for cystic echinococcosis (CE), i.e., a gross tumour composed of multiple large vesicles with several centimeters in diameter. In addition, there were neither extensive adhesions nor infiltrations of the neighboring pancreas and diaphragm as was expected from previous imaging results. The unexpected diagnosis of AE was confirmed by definite histopathology, specific polymerase chain reaction and serology results. This is a rare case of unusual macroscopic presentation of AE that posed immense diagnostic challenges and had an eventful course. To our knowledge this is the first case of an autochthonous infection in this particular geographic area of Germany, the federal state of Saxony. This report may provide new hints for an expanding area of risk for AE and emphasizes the risk of complications in the scope of diagnostic procedures and the limitations of modern radiological imaging.
KW  - hemihepatectomy
KW  - multilocularis
KW  - Germany
KW  - Europe
KW  - liver
KW  - alveolar echinococcosis
KW  - echinococcus multilocularis
KW  - autochthonous infection
KW  - liver resection
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-131525
VL  - 19
IS  - 26
ER  -