TY  - INPR
A1  - Bartfeld, Sina
T1  - Modeling infectious diseases and host-microbe interactions in gastrointestinal organoids
T2  - Developmental Biology
N2  - Advances in stem cell research have allowed the development of 3-dimensional (3D) primary cell cultures termed organoid cultures, as they closely mimic the in vivo organization of different cell lineages. Bridging the gap between 2-dimensional (2D) monotypic cancer cell lines and whole organisms, organoids are now widely applied to model development and disease. Organoids hold immense promise for addressing novel questions in host-microbe interactions, infectious diseases and the resulting inflammatory conditions. Researchers have started to use organoids for modeling infection with pathogens, such as Helicobacter pylori or Salmonella enteritica, gut- microbiota interactions and inflammatory bowel disease. Future studies will broaden the spectrum of microbes used and continue to establish organoids as a standard model for human host-microbial interactions. Moreover, they will increasingly exploit the unique advantages of organoids, for example to address patient-specific responses to microbes.
KW  - gastrointestinal disease
KW  - salmonella
KW  - microbiota
KW  - inflammatory bowel disease
KW  - organoid culture
KW  - helicobacter
KW  - rotavirus
KW  - norovirus
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-138788
UR  - http://www.sciencedirect.com/science/article/pii/S0012160616304602
SN  - 0012-1606
N1  - This is the accepted version of the following article: Bartfeld, Sina, Modeling infectious diseases and host-microbe interactions in gastrointestinal organoids, Developmental Biology, 2016, 420, 2, 262-270. http://dx.doi.org/10.1016/j.ydbio.2016.09.014
ER  - 
TY  - JOUR
A1  - Schlagenhauf, Ulrich
A1  - Jakob, Lena
A1  - Eigenthaler, Martin
A1  - Segerer, Sabine
A1  - Jockel-Schneider, Yvonne
A1  - Rehn, Monika
T1  - Regular consumption of Lactobacillus reuteri-containing lozenges reduces pregnancy gingivitis: an RCT
JF  - Journal of Clinical Periodontology
N2  - Aim: 
This randomized controlled trial assessed the impact of Lactobacillus reuteri on pregnancy gingivitis in healthy women. 

Materials and Methods: 
Forty-five healthy women (24 test/21 placebo) with pregnancy gingivitis in the third trimester of pregnancy were enrolled. At baseline Gingival Index (GI) and Plaque Index (PlI) were assessed at the Ramfjord teeth and venous blood taken for TNF-alpha analysis. Subsequently participants were randomly provided with lozenges to be consumed 2 9 daily until birth (approx. 7 weeks) containing >= 10(8) CFU L. reuteri ATCC PTA 5289 and >= 10(8) CFU L. reuteri DSM 17938 (test) or being devoid of L. reuteri (placebo). Within 2 days after birth recording of GI, PlI and blood sampling were repeated. 

Results: 
At baseline, mean GI and mean PlI did not differ significantly between both groups. In the test group mean TNF-alpha serum level was significantly (p < 0.02) lower than in the placebo group. At reevaluation, mean GI and mean PlI of the test group were both significantly (p < 0.0001) lower than in the placebo group. Mean TNF-alpha serum level did no longer differ significantly between the groups. 

Conclusions: 
The consumption of L. reuteri lozenges may be a useful adjunct in the control of pregnancy gingivitis.
KW  - chronic periodontitis
KW  - probiotic lozenges
KW  - subgingival
KW  - bacteria
KW  - postpartum
KW  - microbiota
KW  - disease
KW  - L. reuteri
KW  - plaque
KW  - pregnancy
KW  - pregnancy gingivitis
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-186783
VL  - 43
IS  - 11
ER  -