TY  - JOUR
A1  - Rockel, Anna F.
A1  - Wagner, Nicole
A1  - Spenger, Peter
A1  - Ergün, Süleyman
A1  - Wörsdörfer, Philipp
T1  - Neuro-mesodermal assembloids recapitulate aspects of peripheral nervous system development \(in\) \(vitro\)
JF  - Stem Cell Reports
N2  - Summary
Here we describe a novel neuro-mesodermal assembloid model that recapitulates aspects of peripheral nervous system (PNS) development such as neural crest cell (NCC) induction, migration, and sensory as well as sympathetic ganglion formation. The ganglia send projections to the mesodermal as well as neural compartment. Axons in the mesodermal part are associated with Schwann cells. In addition, peripheral ganglia and nerve fibers interact with a co-developing vascular plexus, forming a neurovascular niche. Finally, developing sensory ganglia show response to capsaicin indicating their functionality.

The presented assembloid model could help to uncover mechanisms of human NCC induction, delamination, migration, and PNS development. Moreover, the model could be used for toxicity screenings or drug testing. The co-development of mesodermal and neuroectodermal tissues and a vascular plexus along with a PNS allows us to investigate the crosstalk between neuroectoderm and mesoderm and between peripheral neurons/neuroblasts and endothelial cells.

Highlights
•Novel neuro-mesodermal assembloid model of peripheral nervous system development
•Model covers neural crest cell induction, migration, and ganglion formation
•Ganglia send projections to the mesodermal as well as neural compartment
•Peripheral ganglia and nerve fibers interact with a co-developing vascular plexus
KW  - peripheral nervous system
KW  - neural crest
KW  - sensory ganglia
KW  - sensory neuron
KW  - vasculature
KW  - blood vessel
KW  - neural organoid
KW  - mesodermal organoid
KW  - assembloid
KW  - human induced pluripotent stem cells
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-349925
SN  - 2213-6711
VL  - 18
IS  - 5
ER  -