TY  - JOUR
A1  - Pernitzsch, Sandy R.
A1  - Alzheimer, Mona
A1  - Bremer, Belinda U.
A1  - Robbe-Saule, Marie
A1  - De Reuse, Hilde
A1  - Sharma, Cynthia M.
T1  - Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori
JF  - Nature Communications
N2  - The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase variation by also modulating the expression of a gene (hp0102) that is co-transcribed with tlpB. The hp0102 gene encodes a glycosyltransferase required for LPS O-chain biosynthesis and in vivo colonization of the mouse stomach. The G-repeat length defines a gradual (rather than ON/OFF) control of LPS biosynthesis by RepG, and leads to gradual resistance to a membrane-targeting antibiotic. Thus, RepG-mediated modulation of LPS structure might impact host immune recognition and antibiotic sensitivity, thereby helping H. pylori to adapt and persist in the host. The small RNA RepG modulates expression of chemotaxis receptor TlpB in Helicobacter pylori by targeting a length-variable G-repeat in the tlpB mRNA. Here, Pernitzsch et al. show that RepG also gradually controls lipopolysaccharide biosynthesis, antibiotic susceptibility, and in-vivo colonization of the stomach, by regulating a gene that is co-transcribed with tlpB.
KW  - bacterial genetics
KW  - bacterial immune evasion
KW  - pathogens
KW  - small RNAs
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-261536
VL  - 12
IS  - 1
ER  - 
TY  - JOUR
A1  - Pernitzsch, Sandy R.
A1  - Sharma, Cynthia M.
T1  - Transcriptome Complexity and Riboregulation in the Human Pathogen Helicobacter pylori
KW  - Medizin
KW  - RNA-seq
KW  - sRNA
KW  - Helicobacterpylori
KW  - post-transcriptionalregulation
KW  - transcriptomeanalysis
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-75096
ER  - 
TY  - JOUR
A1  - Jäger, Dominik
A1  - Pernitzsch, Sandy R.
A1  - Richter, Andreas S.
A1  - Backofen, Rolf
A1  - Sharma, Cynthia M.
A1  - Schmitz, Ruth A.
T1  - An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains
JF  - Nucleic Acids Research
N2  - We report on the characterization and target analysis of the small (s) RNA\(_{162}\) in the methanoarchaeon Methanosarcina mazei. Using a combination of genetic approaches, transcriptome analysis and computational predictions, the bicistronic MM2441-MM2440 mRNA encoding the transcription factor MM2441 and a protein of unknown function was identified as a potential target of this sRNA, which due to processing accumulates as three stabile 5' fragments in late exponential growth. Mobility shift assays using various mutants verified that the non-structured single-stranded linker region of sRNA\(_{162}\) (SLR) base-pairs with the MM2440-MM2441 mRNA internally, thereby masking the predicted ribosome binding site of MM2441. This most likely leads to translational repression of the second cistron resulting in dis-coordinated operon expression. Analysis of mutant RNAs in vivo confirmed that the SLR of sRNA\(_{162}\) is crucial for target interactions. Furthermore, our results indicate that sRNA\(_{162}\)-controlled MM2441 is involved in regulating the metabolic switch between the carbon sources methanol and methylamine. Moreover, biochemical studies demonstrated that the 50 end of sRNA\(_{162}\) targets the 5'-untranslated region of the cis-encoded MM2442 mRNA. Overall, this first study of archaeal sRNA/mRNA-target interactions unraveled that sRNA\(_{162}\) acts as an antisense (as) RNA on cis- and trans-encoded mRNAs via two distinct domains, indicating that cis-encoded asRNAs can have larger target regulons than previously anticipated.
KW  - strain
KW  - escherichia coli
KW  - methanosarcina mazei GO1
KW  - methanol methyltransferase isozymes
KW  - small nucleolar RNAs
KW  - acetivorans C2A
KW  - antisense RNAs
KW  - GO1
KW  - transcriptional regulator
KW  - translational initiation
KW  - pyrococcus furiosus
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-134972
VL  - 40
IS  - 21
ER  -