TY  - JOUR
A1  - Shan, Junwen
A1  - Böck, Thomas
A1  - Keller, Thorsten
A1  - Forster, Leonard
A1  - Blunk, Torsten
A1  - Groll, Jürgen
A1  - Teßmar, Jörg
T1  - TEMPO/TCC as a Chemo Selective Alternative for the Oxidation of Hyaluronic Acid
JF  - Molecules
N2  - Hyaluronic acid (HA)-based hydrogels are very commonly applied as cell carriers for different approaches in regenerative medicine. HA itself is a well-studied biomolecule that originates from the physiological extracellular matrix (ECM) of mammalians and, due to its acidic polysaccharide structure, offers many different possibilities for suitable chemical modifications which are necessary to control, for example, network formation. Most of these chemical modifications are performed using the free acid function of the polymer and, additionally, lead to an undesirable breakdown of the biopolymer’s backbone. An alternative modification of the vicinal diol of the glucuronic acid is oxidation with sodium periodate to generate dialdehydes via a ring opening mechanism that can subsequently be further modified or crosslinked via Schiff base chemistry. Since this oxidation causes a structural destruction of the polysaccharide backbone, it was our intention to study a novel synthesis protocol frequently applied to selectively oxidize the C6 hydroxyl group of saccharides. On the basis of this TEMPO/TCC oxidation, we studied an alternative hydrogel platform based on oxidized HA crosslinked using adipic acid dihydrazide as the crosslinker.
KW  - hyaluronic acid
KW  - oxidation
KW  - hydrogel formation
KW  - Schiff base chemistry
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-248362
SN  - 1420-3049
VL  - 26
IS  - 19
ER  - 
TY  - JOUR
A1  - Horder, Hannes
A1  - Guaza Lasheras, Mar
A1  - Grummel, Nadine
A1  - Nadernezhad, Ali
A1  - Herbig, Johannes
A1  - Ergün, Süleyman
A1  - Teßmar, Jörg
A1  - Groll, Jürgen
A1  - Fabry, Ben
A1  - Bauer-Kreisel, Petra
A1  - Blunk, Torsten
T1  - Bioprinting and differentiation of adipose-derived stromal cell spheroids for a 3D breast cancer-adipose tissue model
JF  - Cells
N2  - Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such as in cancer research. In breast cancer, adipose tissue has been acknowledged as an important part of the tumor microenvironment favoring tumor progression. Therefore, in this study, a 3D-printed breast cancer model for facilitating investigations into cancer cell-adipocyte interaction was developed. First, we focused on the printability of human adipose-derived stromal cell (ASC) spheroids in an extrusion-based bioprinting setup and the adipogenic differentiation within printed spheroids into adipose microtissues. The printing process was optimized in terms of spheroid viability and homogeneous spheroid distribution in a hyaluronic acid-based bioink. Adipogenic differentiation after printing was demonstrated by lipid accumulation, expression of adipogenic marker genes, and an adipogenic ECM profile. Subsequently, a breast cancer cell (MDA-MB-231) compartment was printed onto the adipose tissue constructs. After nine days of co-culture, we observed a cancer cell-induced reduction of the lipid content and a remodeling of the ECM within the adipose tissues, with increased fibronectin, collagen I and collagen VI expression. Together, our data demonstrate that 3D-printed breast cancer-adipose tissue models can recapitulate important aspects of the complex cell–cell and cell–matrix interplay within the tumor-stroma microenvironment
KW  - adipose-derived stromal cells
KW  - adipose tissue
KW  - bioprinting
KW  - breast cancer model
KW  - extracellular matrix
KW  - hyaluronic acid
KW  - spheroids
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-236496
VL  - 10
IS  - 4
ER  -