TY - JOUR A1 - Lamatsch, D. K. A1 - Trifonov, V. A1 - Schories, S. A1 - Epplen, J. T. A1 - Schmid, M. A1 - Schartl, M. T1 - Isolation of a Cancer-Associated Microchromosome in the Sperm-Dependent Parthenogen Poecilia formosa JF - Cytogenetic and Genome Research N2 - In the asexual all-female fish species Poecilia formosa, the Amazon molly, supernumerary chromosomes have frequently been found in both laboratory-reared and wild-caught individuals. While wild-caught individuals with B chromosomes are phenotypically indifferent from conspecifics, individuals carrying B chromosomes from recent introgression events in the laboratory show phenotypic changes. Former analyses showed that the expression of a pigment cell locus is associated with the presence of these B chromosomes. In addition, they contain a so far unidentified locus that confers a higher susceptibility to tumor formation in the presence of pigmentation pattern. Isolation by microdissection and hybridization to metaphase chromosomes revealed that they contain one or several sequences with similarity to a highly repetitive pericentromeric and subtelomeric sequence in A chromosomes. Isolation of one particular sequence by AFLP showed that the B chromosomes contain at least 1 copy of an A-chromosomal region which is highly conserved in the whole genus Poecilia, i.e. more than 5 million years old. We propose it to be a single copy sequence. KW - paternal introgression KW - AFLP KW - asexual reproduction KW - B chromosomes KW - gynogenesis KW - microdissection KW - telomeres Y1 - 2011 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-196785 SN - 1424-8581 SN - 1424-859X N1 - This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. VL - 135 IS - 2 ER - TY - JOUR A1 - Lubjuhn, T. A1 - Schartl, Manfred A1 - Epplen, J. T. T1 - Methodik und Anwendungsgebiete des genetischen Fingerabdruckverfahrens N2 - No abstract available KW - Physiologische Chemie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61978 ER - TY - CHAP A1 - Schartl, Manfred A1 - Erbelding-Denk, C. A1 - Hölter, S. A1 - Nanda, I. A1 - Schmid, M. A1 - Schröder, J. H. A1 - Epplen, J. T. T1 - High mating success of low rank males in Limia perugiae (Pisces: Poeciliidae) as determined by DNA-fingerprinting N2 - Hierarchical structures among male individuals in a population are frequently reflected in differences in aggressive and reproductive behaviour and access to the females. In general social dominance requires large investments which in turn may have to be compensated for by high reproductive success. However, this hypothesis has so far only been sufficiently tested in small mating groups due to the difficulties of determining paternity by classical methods using non-molecular markers. DNA fingerprinting overcomes these problems offering the possibility to determine genetic relationships and mating patterns within larger groups. Using this approach we have recently shown (Schartl et al., 1993) that in the poeciliid fish Limia perugiae in small mating groups the dominant male has 100% mating success, while in larger groups its contribution to the offspring unexpectedly drops to zero. The reproductive failure under such social conditions is explained by the inability of the ex-male to protect all the females simultaneously against mating attempts of his numerous subordinate competitors. KW - DNS KW - Fingerprint-Verfahren KW - Lebendgebärende Zahnkarpfen Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87132 ER - TY - CHAP A1 - Epplen, J. T. A1 - Ammer, H. A1 - Epplen, C. A1 - Kammerbauer, C. A1 - Mitreiter, R. A1 - Roewer, L. A1 - Schwaiger, W. A1 - Steimle, V. A1 - Zischler, H. A1 - Albert, E. A1 - Andreas, A. A1 - Beyermann, B. A1 - Meyer, W. A1 - Buitkamp, J. A1 - Nanda, I. A1 - Nürnberg, P. A1 - Pena, S. D. J. A1 - Pöche, H. A1 - Sprecher, W. A1 - Schartl, Manfred A1 - Weising, K. A1 - Yassouridis, A. T1 - Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect hypervariability for multiple purposes N2 - A panel of simple repetitive oligonucleotide probes has been designed and tested for multilocus DNA fingerprinting in some 200 fungal, plant and animal species as well as man. To date at least one of the probes has been found to be informative in each species. The human genome, however, has been the major target of many fingerprintins studies. Using the probe (CAC)5 or (GTG)5, individualization of all humans is possible except for monozygotic twins. Paternity analyses are now perfonned on a routine basis by the use of multilocus fingerprints, inctuding also cases of deficiency, i.e. where one of the parents is not available for analysis. In forensie science stain analysis is feasible in all tissue remains containing nuc)eated cells. Depending on the degree of DNA degradation a variety of oligonucleotides are informative, and they have been proven useful in actual case work. Advantages in comparison to other methods including enzymatic DNA amplification techniques (PCR) are evident. Fingerprint patterns of tumors may be changed due to the gain or loss of chromosomes and/or intrachromosomal deletion and amplification events. Locus-specific probes were isolated from the human (CAC)5/( GTG)5 fingerprint with a varying degree of informativeness (monomorphic versus truly hypervariable markers). The feasibility of three different approaches. for the isolation of hypervariable mono-locus probes was evaluated. Finally, one particular mixed simple (gt)n(ga)m repeat locus in the second intron of the HLA-DRB genes has been scrutinized to allow comparison of the extent of exon-encoded (protein-) polymorphisms versus intronie bypervariability of simple repeats: adjacent to a single gene sequence (e.g. HLA-DRB1*0401) many different length alleles were found. Group-specific structures of basic repeats were identified within the evolutionarily related DRB alleles. As a further application it is suggested here that due to the ubiquitous interspersion of their targets, short probes for simple repeat sequences are especially useful tools for ordering genomic cosmid, yeast artificial chromosome and phage banks. KW - DNS KW - Fingerprint-Verfahren Y1 - 1991 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86371 ER - TY - JOUR A1 - Weising, K. A1 - Fiala, Brigitte A1 - Ramloch, K. A1 - Kahl, K. A1 - Epplen, J. T. T1 - Olingonucleotide fingerprinting in angiosperms N2 - No abstract available Y1 - 1990 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-42884 ER -