TY - JOUR A1 - Bauriedl, Saskia A1 - Gerovac, Milan A1 - Heidrich, Nadja A1 - Bischler, Thorsten A1 - Barquist, Lars A1 - Vogel, Jörg A1 - Schoen, Christoph T1 - The minimal meningococcal ProQ protein has an intrinsic capacity for structure-based global RNA recognition JF - Nature Communications N2 - FinO-domain proteins are a widespread family of bacterial RNA-binding proteins with regulatory functions. Their target spectrum ranges from a single RNA pair, in the case of plasmid-encoded FinO, to global RNA regulons, as with enterobacterial ProQ. To assess whether the FinO domain itself is intrinsically selective or promiscuous, we determine in vivo targets of Neisseria meningitidis, which consists of solely a FinO domain. UV-CLIP-seq identifies associations with 16 small non-coding sRNAs and 166 mRNAs. Meningococcal ProQ predominantly binds to highly structured regions and generally acts to stabilize its RNA targets. Loss of ProQ alters transcript levels of >250 genes, demonstrating that this minimal ProQ protein impacts gene expression globally. Phenotypic analyses indicate that ProQ promotes oxidative stress resistance and DNA damage repair. We conclude that FinO domain proteins recognize some abundant type of RNA shape and evolve RNA binding selectivity through acquisition of additional regions that constrain target recognition. FinO-domain proteins are bacterial RNA-binding proteins with a wide range of target specificities. Here, the authors employ UV CLIP-seq and show that minimal ProQ protein of Neisseria meningitidis binds to various small non-coding RNAs and mRNAs involved in virulence. KW - Neisseria meningitidis KW - natural transformation KW - dual function KW - FinO family KW - HFQ KW - chaperone KW - transcriptome KW - regulator KW - sequence KW - in vivo Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-230040 VL - 11 ER - TY - JOUR A1 - Palige, Katja A1 - Linde, Jörg A1 - Martin, Ronny A1 - Böttcher, Bettina A1 - Citiulo, Francesco A1 - Sullivan, Derek J. A1 - Weber, Johann A1 - Staib, Claudia A1 - Rupp, Steffen A1 - Hube, Bernhard A1 - Morschhäuser, Joachim A1 - Staib, Peter T1 - Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis JF - PLoS ONE N2 - Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens. KW - NRG1 KW - staib agar KW - gene KW - morphogenesis KW - expression KW - regulator KW - virulence KW - growth KW - UME6 KW - epidemiology Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-131007 VL - 8 IS - 4 ER - TY - THES A1 - Joachim, Silvia T1 - Regulatorketten in Butlergruppen T1 - Regulator Chains of Butler Groups N2 - Die fast vollständig zerlegbaren Gruppen bilden eine Teilklasse der Butlergruppen. Das Konzept des Regulators, d.h. der Durchschnitt aller regulierenden Untergruppen, ist unverzichtbar für fast vollständig zerlegbare Gruppen. Dieses Konzept lässt sich in natürlicher Weise auf die ganze Klasse der Butlergruppen fortsetzen. Allerdings lässt sich die Regulatorbildung im allgemeineren Fall der Butlergruppen a priori iterieren. Damit stellt sich erst einmal die Frage, ob es überhaupt Butlergruppen gibt mit Regulatorketten, der Länge größer als 1. Ein erstes Beispiel der Länge 2 wurde 1997 von Lehrmann und Mutzbauer konstruiert. In dieser Dissertation wurden mit konzeptionell neuen Techniken Butlergruppen mit beliebiger vorgegebener endlicher Kettenlänge angegeben. Grundsätzliche Schwierigkeiten bei diesem Unterfangen resultieren aus dem Fehlen, bzw. der Unmöglichkeit, einer kanonischen Darstellung von Butlergruppen. Man verwendet die allseits gebrauchte Summendarstellung für Butlergruppen. Genau an dieser Stelle bedarf es völlig neuer Methoden, verglichen mit den fast vollständig zerlegbaren Gruppen mit ihrer kanonischen Regulatordarstellung. Alle Teilaufgaben bei der anstehenden Konstruktion von Butlergruppen, die für fast vollständig zerlegbare Gruppen Standard sind, werden hierbei problematisch, u.a. die Bildung reiner Hüllen, die Bestimmung regulierender Untergruppen und die Regulatorbildung. N2 - The almost completely decomposable groups form a subclass of the Butler groups. The concept of a regulator, i. e., the intersection of all regulating subgroups, is inevitable for almost completely decomposable groups. This concept can be transferred and continued to the whole class of Butler groups in a natural way. However, forming the regulator for Butler groups usually allows proper iteration. Thus, the primary question is, if there are any Butler groups at all with longer regulator chains, the length longer than 1. A first example of length 2 was constructed by Lehrmann and Mutzbauer in 1997. In this doctoral dissertation Butler groups were constructed of an arbitrarily given finite chain length, using conceptually new techniques. Basic difficulties resulted from the lack, or respectively, the impossibility, of any canonical descriptions of Butler groups. Usually Butler groups are given by the so called sum representation. Precisely here completely new methods are necessary to be applied, compared with the almost completely decomposable groups and their canonical regulator representation. All detailed tasks for the indicated construction of Butler groups, which are standard for almost completely decomposable groups, become problematic, among other things the forming of pure hulls, the determination of regulating subgroups, and the construction of the regulator. KW - Butlergruppe KW - Regulator KW - Butlergruppe KW - regulierende Untergruppen KW - Regulator KW - Butler group KW - regulating subgroup KW - regulator Y1 - 2004 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-10438 ER - TY - THES A1 - Dittmann, Ulrich T1 - Coset Types and Tight Subgroups of Almost Completely Decomposable Groups T1 - Nebenklassentypen und tight Untergruppen von fast vollständig zerlegbaren Gruppen N2 - A completely decomposable group is a direct sum of subgroups of the rationals. An almost completely decomposable group is a torsion free abelian group that contains a completely decomposable group as subgroup of finite index. Tight subgroups are maximal subgroups (with respect to set inclusion) among the completely decomposable subgroups of an almost completely decomposable group. In this dissertation we show an extended version of the theorem of Bezout, give a new criterion for the tightness of a completely decomposable subgroup, derive some conditions under which a tight subgroup is regulating and generalize a theorem of Campagna. We give an example of an almost completely decomposable group, all of whose regulating subgroups do not have a quotient with minimal exponent. We show that among the types of elements of a coset modulo a completely decomposable group there exists a unique maximal type and define this type to be -the- coset type. We give criteria for tightness and regulating in term of coset types as well as a representation of the type subgroups using coset types. We introduce the notion of reducible cosets and show their key role for transitions from one completely decomposable subgroup up to another one containing the first one as a proper subgroup. We give an example of a tight, but not regulating subgroup which contains the regulator. We develop the notion of a fully single covered subset of a lattice, show that V-free implies fully single covered, but not necessarily vice versa, and we define an equivalence relation on the set of all finite subsets of a given lattice. We develop some extension of ordinary Hasse diagrams, and apply the lattice theoretic results on the lattice of types and almost completely decomposable groups. N2 - Eine vollständig zerlegbare Gruppe ist eine direkte Summe von Untergruppen der rationalen Zahlen. Eine fast vollständig zerlegbare Gruppe ist eine torsionsfreie abelsche Gruppe, die eine vollständig zerlegbare Gruppe als Untergruppe von endlichem Index enthält. Tight Untergruppen sind bezüglich Mengeninklusion maximale Elemente der Menge der vollständig zerlegbaren Untergruppen einer fast vollständig zerlegbaren Gruppe. In dieser Dissertation zeigen wir eine erweiterte Version des Satzes von Bezout, geben ein neues Kriterium an, mit dem festgestellt werden kann, ob eine Untergruppe tight ist, leiten daraus einige Bedingungen ab, unter denen eine tight Untergruppe regulierend ist, und verallgemeinern einen Satz von Campagna. Wir geben ein Beispiel einer fast vollständig zerlegbaren Gruppe an, deren sämtliche regulierende Untergruppen nicht minimalen Exponenten des Quotienten haben. Wir zeigen, daß unter den Typen der Elemente einer Nebenklasse modulo einer vollständig zerlegbaren Gruppe ein eindeutig definierter maximaler Typ existiert und nennen diesen Typen -den- Nebenklassentypen. Wir geben Kriterien für tight und regulierend mit Hilfe von Nebenklassentypen, sowie eine Darstellung der Typenuntergruppen. Wir führen den Begriff der reduziblen Nebenklassen ein und zeigen die Schlüsselrolle, die diese beim Übergang von einer vollständig zerlegbaren Untergruppe zu einer anderen, die die erste enthält, haben. Wir geben ein Beispiel einer tight Untergruppe an, die nicht regulierend ist, aber den Regulator enthält. Wir führen den Begriff einer "fully single covered" Untermenge eines Verbandes ein, zeigen daß V-frei "fully single covered" impliziert, aber nicht umgekehrt, und definieren eine Äquivalenzrelation auf der Menge aller endlichen Untermengen eines Verbandes. Wir entwickeln eine Erweiterung der üblichen Hasse Diagramme und wenden die verbandstheoretischen Ergebnisse auf die Typenmenge fast vollständig zerlegbarer Gruppen an. KW - Torsionsfreie Abelsche Gruppe KW - Untergruppe KW - Restklasse KW - Regulator KW - fast vollständig zerlegbare Gruppe KW - tight KW - regulierend KW - Regulator KW - almost completely decomposable group KW - tight KW - regulating KW - regulator Y1 - 2001 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-2762 ER -