TY  - JOUR
A1  - Hurd, Paul J.
A1  - Grübel, Kornelia
A1  - Wojciechowski, Marek
A1  - Maleszka, Ryszard
A1  - Rössler, Wolfgang
T1  - Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
JF  - Scientific Reports
N2  - In the course of a screen designed to produce antibodies (ABs) with affinity to proteins in the honey bee brain we found an interesting AB that detects a highly specific epitope predominantly in the nuclei of Kenyon cells (KCs). The observed staining pattern is unique, and its unfamiliarity indicates a novel previously unseen nuclear structure that does not colocalize with the cytoskeletal protein f-actin. A single rod-like assembly, 3.7-4.1 mu m long, is present in each nucleus of KCs in adult brains of worker bees and drones with the strongest immuno-labelling found in foraging bees. In brains of young queens, the labelling is more sporadic, and the rod-like structure appears to be shorter (similar to 2.1 mu m). No immunostaining is detectable in worker larvae. In pupal stage 5 during a peak of brain development only some occasional staining was identified. Although the cellular function of this unexpected structure has not been determined, the unusual distinctiveness of the revealed pattern suggests an unknown and potentially important protein assembly. One possibility is that this nuclear assembly is part of the KCs plasticity underlying the brain maturation in adult honey bees. Because no labelling with this AB is detectable in brains of the fly Drosophila melanogaster and the ant Camponotus floridanus, we tentatively named this antibody AmBNSab (Apis mellifera Brain Neurons Specific antibody). Here we report our results to make them accessible to a broader community and invite further research to unravel the biological role of this curious nuclear structure in the honey bee central brain.
KW  - mushroom body calyx
KW  - synaptic complexes
KW  - bodies
KW  - insect
KW  - plasticity
KW  - insights
KW  - genome
KW  - model
KW  - proteins
KW  - methylation
KW  - biological techniques
KW  - cell biology
KW  - developmental biology
KW  - molecular biology
KW  - neuroscience
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-260059
VL  - 11
ER  - 
TY  - JOUR
A1  - Pauli, Martin
A1  - Paul, Mila M.
A1  - Proppert, Sven
A1  - Mrestani, Achmed
A1  - Sharifi, Marzieh
A1  - Repp, Felix
A1  - Kürzinger, Lydia
A1  - Kollmannsberger, Philip
A1  - Sauer, Markus
A1  - Heckmann, Manfred
A1  - Sirén, Anna-Leena
T1  - Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections
JF  - Communications Biology
N2  - Revealing the molecular organization of anatomically precisely defined brain regions is necessary for refined understanding of synaptic plasticity. Although three-dimensional (3D) single-molecule localization microscopy can provide the required resolution, imaging more than a few micrometers deep into tissue remains challenging. To quantify presynaptic active zones (AZ) of entire, large, conditional detonator hippocampal mossy fiber (MF) boutons with diameters as large as 10 mu m, we developed a method for targeted volumetric direct stochastic optical reconstruction microscopy (dSTORM). An optimized protocol for fast repeated axial scanning and efficient sequential labeling of the AZ scaffold Bassoon and membrane bound GFP with Alexa Fluor 647 enabled 3D-dSTORM imaging of 25 mu m thick mouse brain sections and assignment of AZs to specific neuronal substructures. Quantitative data analysis revealed large differences in Bassoon cluster size and density for distinct hippocampal regions with largest clusters in MF boutons. Pauli et al. develop targeted volumetric dSTORM in order to image large hippocampal mossy fiber boutons (MFBs) in brain slices. They can identify synaptic targets of individual MFBs and measured size and density of Bassoon clusters within individual untruncated MFBs at nanoscopic resolution.
KW  - mossy fiber synapses
KW  - CA3 pyrimidal cells
KW  - CA2+ channels
KW  - active zone
KW  - hippocampal
KW  - release
KW  - plasticity
KW  - proteins
KW  - platform
KW  - reveals
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-259830
VL  - 4
ER  -