TY  - JOUR
A1  - Xu, Jietao
A1  - Fahmy-Garcia, Shorouk
A1  - Wesdorp, Marinus A.
A1  - Kops, Nicole
A1  - Forte, Lucia
A1  - De Luca, Claudio
A1  - Misciagna, Massimiliano Maraglino
A1  - Dolcini, Laura
A1  - Filardo, Giuseppe
A1  - Labberté, Margot
A1  - Vancíková, Karin
A1  - Kok, Joeri
A1  - van Rietbergen, Bert
A1  - Nickel, Joachim
A1  - Farrell, Eric
A1  - Brama, Pieter A. J.
A1  - van Osch, Gerjo J. V. M.
T1  - Effectiveness of BMP-2 and PDGF-BB adsorption onto a collagen/collagen-magnesium-hydroxyapatite scaffold in weight-bearing and non-weight-bearing osteochondral defect bone repair: in vitro, ex vivo and in vivo evaluation
JF  - Journal of Functional Biomaterials
N2  - Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet-derived growth factor-BB (PDGF-BB) onto said scaffold. The in vitro release kinetics of BMP-2/PDGF-BB demonstrated that PDGF-BB was burst released from the collagen-only layer, whereas BMP-2 was largely retained in both layers. Cell ingrowth was enhanced by BMP-2/PDFG-BB in a bovine osteochondral defect ex vivo model. In an in vivo semi-orthotopic athymic mouse model, adding BMP-2 or PDGF-BB increased tissue repair after four weeks. After eight weeks, most defects were filled with bone tissue. To further investigate the promising effect of BMP-2, a caprine bilateral stifle osteochondral defect model was used where defects were created in weight-bearing femoral condyle and non-weight-bearing trochlear groove locations. After six months, the adsorption of BMP-2 resulted in significantly less bone repair compared with scaffold-only in the femoral condyle defects and a trend to more bone repair in the trochlear groove. Overall, the adsorption of BMP-2 onto a Col/Col-Mg-HAp scaffold reduced bone formation in weight-bearing osteochondral defects, but not in non-weight-bearing osteochondral defects.
KW  - tissue engineering
KW  - regenerative medicine
KW  - osteochondral lesion
KW  - biocompatible materials
KW  - bone morphogenetic proteins
KW  - platelet-derived growth factor
KW  - animal model
KW  - weight-bearing
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304019
SN  - 2079-4983
VL  - 14
IS  - 2
ER  -