TY  - JOUR
A1  - Soundararajan, Manonmani
A1  - Marincola, Gabriella
A1  - Liong, Olivia
A1  - Marciniak, Tessa
A1  - Wencker, Freya D. R.
A1  - Hofmann, Franka
A1  - Schollenbruch, Hannah
A1  - Kobusch, Iris
A1  - Linnemann, Sabrina
A1  - Wolf, Silver A.
A1  - Helal, Mustafa
A1  - Semmler, Torsten
A1  - Walther, Birgit
A1  - Schoen, Christoph
A1  - Nyasinga, Justin
A1  - Revathi, Gunturu
A1  - Boelhauve, Marc
A1  - Ziebuhr, Wilma
T1  - Farming practice influences antimicrobial resistance burden of non-aureus staphylococci in pig husbandries
JF  - Microorganisms
N2  - Non-aureus staphylococci (NAS) are ubiquitous bacteria in livestock-associated environments where they may act as reservoirs of antimicrobial resistance (AMR) genes for pathogens such as Staphylococcus aureus. Here, we tested whether housing conditions in pig farms could influence the overall AMR-NAS burden. Two hundred and forty porcine commensal and environmental NAS isolates from three different farm types (conventional, alternative, and organic) were tested for phenotypic antimicrobial susceptibility and subjected to whole genome sequencing. Genomic data were analysed regarding species identity and AMR gene carriage. Seventeen different NAS species were identified across all farm types. In contrast to conventional farms, no AMR genes were detectable towards methicillin, aminoglycosides, and phenicols in organic farms. Additionally, AMR genes to macrolides and tetracycline were rare among NAS in organic farms, while such genes were common in conventional husbandries. No differences in AMR detection existed between farm types regarding fosfomycin, lincosamides, fusidic acid, and heavy metal resistance gene presence. The combined data show that husbandry conditions influence the occurrence of resistant and multidrug-resistant bacteria in livestock, suggesting that changing husbandry practices may be an appropriate means of limiting the spread of AMR bacteria on farms.
KW  - non-aureus staphylococci
KW  - NAS
KW  - alternative pig farming
KW  - antimicrobial resistance
KW  - one-health approach
KW  - intervention strategies
KW  - livestock-associated staphylococci
KW  - organic farming
KW  - pig farming methods
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-312750
SN  - 2076-2607
VL  - 11
IS  - 1
ER  - 
TY  - JOUR
A1  - Körner, Maria
A1  - Meyer, Susanne R.
A1  - Marincola, Gabriella
A1  - Kern, Maximilian J.
A1  - Grimm, Clemens
A1  - Schuelein-Voelk, Christina
A1  - Fischer, Utz
A1  - Hofmann, Kay
A1  - Buchberger, Alexander
T1  - The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP
JF  - eLife
N2  - The ATPase p97 (also known as VCP, Cdc48) has crucial functions in a variety of important cellular processes such as protein quality control, organellar homeostasis, and DNA damage repair, and its de-regulation is linked to neuromuscular diseases and cancer. p97 is tightly controlled by numerous regulatory cofactors, but the full range and function of the p97–cofactor network is unknown. Here, we identify the hitherto uncharacterized FAM104 proteins as a conserved family of p97 interactors. The two human family members VCP nuclear cofactor family member 1 and 2 (VCF1/2) bind p97 directly via a novel, alpha-helical motif and associate with p97-UFD1-NPL4 and p97-UBXN2B complexes in cells. VCF1/2 localize to the nucleus and promote the nuclear import of p97. Loss of VCF1/2 results in reduced nuclear p97 levels, slow growth, and hypersensitivity to chemical inhibition of p97 in the absence and presence of DNA damage, suggesting that FAM104 proteins are critical regulators of nuclear p97 functions.
KW  - p97 VCP Cdc48
KW  - ubiquitin proteasome system
KW  - nuclear import
KW  - DNA damage repair
KW  - FAM104A
KW  - FLJ14775
KW  - FAM104B
KW  - FLJ20434
KW  - CXorf44
KW  - cell biology
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-350222
VL  - 12
ER  -