TY  - JOUR
A1  - Goeritzer, Madeleine
A1  - Kuentzel, Katharina B.
A1  - Beck, Sarah
A1  - Korbelius, Melanie
A1  - Rainer, Silvia
A1  - Bradić, Ivan
A1  - Kolb, Dagmar
A1  - Mussbacher, Marion
A1  - Schrottmaier, Waltraud C.
A1  - Assinger, Alice
A1  - Schlagenhauf, Axel
A1  - Rost, René
A1  - Gottschalk, Benjamin
A1  - Eichmann, Thomas O.
A1  - Züllig, Thomas
A1  - Graier, Wolfgang F.
A1  - Vujić, Nemanja
A1  - Kratky, Dagmar
T1  - Monoglyceride lipase deficiency is associated with altered thrombogenesis in mice
JF  - International Journal of Molecular Sciences
N2  - Monoglyceride lipase (MGL) hydrolyzes monoacylglycerols (MG) to glycerol and one fatty acid. Among the various MG species, MGL also degrades 2-arachidonoylglycerol, the most abundant endocannabinoid and potent activator of the cannabinoid receptors 1 and 2. We investigated the consequences of MGL deficiency on platelet function using systemic (Mgl\(^{−/−}\)) and platelet-specific Mgl-deficient (platMgl\(^{−/−}\)) mice. Despite comparable platelet morphology, loss of MGL was associated with decreased platelet aggregation and reduced response to collagen activation. This was reflected by reduced thrombus formation in vitro, accompanied by a longer bleeding time and a higher blood volume loss. Occlusion time after FeCl\(_3\)-induced injury was markedly reduced in Mgl\(^{−/−}\) mice, which is consistent with contraction of large aggregates and fewer small aggregates in vitro. The absence of any functional changes in platelets from platMgl\(^{−/−}\) mice is in accordance with lipid degradation products or other molecules in the circulation, rather than platelet-specific effects, being responsible for the observed alterations in Mgl\(^{−/−}\) mice. We conclude that genetic deletion of MGL is associated with altered thrombogenesis.
KW  - platelets
KW  - MGL
KW  - in vitro and in vivo thrombus formation
KW  - platelet activation
KW  - platelet aggregation
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304052
SN  - 1422-0067
VL  - 24
IS  - 4
ER  -