TY  - JOUR
A1  - Kosnopfel, Corinna
A1  - Sinnberg, Tobias
A1  - Sauer, Birgit
A1  - Niessner, Heike
A1  - Muenchow, Alina
A1  - Fehrenbacher, Birgit
A1  - Schaller, Martin
A1  - Mertens, Peter R.
A1  - Garbe, Claus
A1  - Thakur, Basant Kumar
A1  - Schittek, Birgit
T1  - Tumour progression stage-dependent secretion of YB-1 stimulates melanoma cell migration and invasion
JF  - Cancers
N2  - Secreted factors play an important role in intercellular communication. Therefore, they are not only indispensable for the regulation of various physiological processes but can also decisively advance the development and progression of tumours. In the context of inflammatory disease, Y-box binding protein 1 (YB-1) is actively secreted and the extracellular protein promotes cell proliferation and migration. In malignant melanoma, intracellular YB-1 expression increases during melanoma progression and represents an unfavourable prognostic marker. Here, we show active secretion of YB-1 from melanoma cells as opposed to benign cells of the skin. Intriguingly, YB-1 secretion correlates with the stage of melanoma progression and depends on a calcium- and ATP-dependent non-classical secretory pathway leading to the occurrence of YB-1 in the extracellular space as a free protein. Along with an elevated YB-1 secretion of melanoma cells in the metastatic growth phase, extracellular YB-1 exerts a stimulating effect on melanoma cell migration, invasion, and tumourigenicity. Collectively, these data suggest that secreted YB-1 plays a functional role in melanoma cell biology, stimulating metastasis, and may serve as a novel biomarker in malignant melanoma that reflects tumour aggressiveness.
KW  - melanoma
KW  - secretion
KW  - Y-box binding protein 1
KW  - migration and invasiveness
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-211206
SN  - 2072-6694
VL  - 12
IS  - 8
ER  -