TY  - JOUR
A1  - Watermann, Christoph
A1  - Meyer, Malin Tordis
A1  - Wagner, Steffen
A1  - Wittekindt, Claus
A1  - Klussmann, Jens Peter
A1  - Erguen, Sueleyman
A1  - Baumgart-Vogt, Eveline
A1  - Karnati, Srikanth
T1  - Peroxisomes are highly abundant and heterogeneous in human parotid glands
JF  - International Journal of Molecular Sciences
N2  - The parotid gland is one of the major salivary glands producing a serous secretion, and it plays an essential role in the digestive and immune systems. Knowledge of peroxisomes in the human parotid gland is minimal; furthermore, the peroxisomal compartment and its enzyme composition in the different cell types of the human parotid gland have never been subjected to a detailed investigation. Therefore, we performed a comprehensive analysis of peroxisomes in the human parotid gland’s striated duct and acinar cells. We combined biochemical techniques with various light and electron microscopy techniques to determine the localization of parotid secretory proteins and different peroxisomal marker proteins in parotid gland tissue. Moreover, we analyzed the mRNA of numerous gene encoding proteins localized in peroxisomes using real-time quantitative PCR. The results confirm the presence of peroxisomes in all striated duct and acinar cells of the human parotid gland. Immunofluorescence analyses for various peroxisomal proteins showed a higher abundance and more intense staining in striated duct cells compared to acinar cells. Moreover, human parotid glands comprise high quantities of catalase and other antioxidative enzymes in discrete subcellular regions, suggesting their role in protection against oxidative stress. This study provides the first thorough description of parotid peroxisomes in different parotid cell types of healthy human tissue.
KW  - peroxisomes
KW  - parotid gland
KW  - human
KW  - catalase
KW  - differential expression
KW  - PSP
KW  - mRNA
KW  - immunofluorescence
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-311079
SN  - 1422-0067
VL  - 24
IS  - 5
ER  - 
TY  - JOUR
A1  - Gredic, Marija
A1  - Karnati, Srikanth
A1  - Ruppert, Clemens
A1  - Guenther, Andreas
A1  - Avdeev, Sergey N.
A1  - Kosanovic, Djuro
T1  - Combined pulmonary fibrosis and emphysema: when Scylla and Charybdis ally
JF  - Cells
N2  - Combined pulmonary fibrosis and emphysema (CPFE) is a recently recognized syndrome that, as its name indicates, involves the existence of both interstitial lung fibrosis and emphysema in one individual, and is often accompanied by pulmonary hypertension. This debilitating, progressive condition is most often encountered in males with an extensive smoking history, and is presented by dyspnea, preserved lung volumes, and contrastingly impaired gas exchange capacity. The diagnosis of the disease is based on computed tomography imaging, demonstrating the coexistence of emphysema and interstitial fibrosis in the lungs, which might be of various types and extents, in different areas of the lung and several relative positions to each other. CPFE bears high mortality and to date, specific and efficient treatment options do not exist. In this review, we will summarize current knowledge about the clinical attributes and manifestations of CPFE. Moreover, we will focus on pathophysiological and pathohistological lung phenomena and suspected etiological factors of this disease. Finally, since there is a paucity of preclinical research performed for this particular lung pathology, we will review existing animal studies and provide suggestions for the development of additional in vivo models of CPFE syndrome.
KW  - CPFE
KW  - lung fibrosis
KW  - emphysema
KW  - animal models
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-313571
SN  - 2073-4409
VL  - 12
IS  - 9
ER  -