TY  - JOUR
A1  - Dashti, Yousef
A1  - Grkovic, Tanja
A1  - Abdelmohsen, Usama Ramadan
A1  - Hentschel, Ute
A1  - Quinn, Ronald J.
T1  - Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp EG49 and Nocardiopsis sp RV163
JF  - MARINE DRUGS
N2  - Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by H-1 NMR. Ten known compounds, including angucycline, diketopiperazine and beta-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a, 6,11a, 12-tetrahydro-5a, 11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.
KW  - co-cultivation
KW  - induced metabolites
KW  - sponge-associated actinomyetes
KW  - NMR fingerprint
KW  - bioactivity
KW  - natural products
KW  - A-D
KW  - aspergillus fumigatus
KW  - marine
KW  - biosynthesis
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116547
SN  - 1660-3397
VL  - 12
IS  - 5
ER  - 
TY  - JOUR
A1  - Kunz, Anna Lena
A1  - Labes, Antje
A1  - Wiese, Jutta
A1  - Bruhn, Torsten
A1  - Bringmann, Gerhard
A1  - Imhoff, Johannes F.
T1  - Nature's Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain
JF  - Marine Drugs
N2  - Eight streptophenazines (A-H) have been identified so far as products of Streptomyces strain HB202, which was isolated from the sponge Halichondria panicea from the Baltic Sea. The variation of bioactivities based on small structural changes initiated further studies on new derivatives. Three new streptophenazines (I-K) were identified after fermentation in the present study. In addition, revised molecular structures of streptophenazines C, D, F and H are proposed. Streptophenazines G and K exhibited moderate antibacterial activity against the facultative pathogenic bacterium Staphylococcus epidermidis and against Bacillus subtilis. All tested compounds (streptophenazines G, I-K) also showed moderate activities against PDE 4B.
KW  - marine natural product
KW  - phenazine
KW  - Baltic Sea
KW  - streptomyces
KW  - halichondria panicea
KW  - obstructive pulmonary disease
KW  - phosphodiesterase-4 inhibitor
KW  - absolute configuration
KW  - biosynthesis
KW  - rofumilast
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116816
SN  - 1660-3397
VL  - 12
IS  - 4
ER  -