TY  - JOUR
A1  - Plauth, Annabell
A1  - Geikowski, Anne
A1  - Cichon, Susanne
A1  - Wowro, Sylvia J.
A1  - Liedgens, Linda
A1  - Rousseau, Morten
A1  - Weidner, Christopher
A1  - Fuhr, Luise
A1  - Kliem, Magdalena
A1  - Jenkins, Gail
A1  - Lotito, Silvina
A1  - Wainwright, Linda J.
A1  - Sauer, Sascha
T1  - Hormetic shifting of redox environment by pro-oxidative resveratrol protects cells against stress
JF  - Free Radical Biology and Medicine
N2  - Resveratrol has gained tremendous interest owing to multiple reported health-beneficial effects. However, the underlying key mechanism of action of this natural product remained largely controversial. Here, we demonstrate that under physiologically relevant conditions major biological effects of resveratrol can be attributed to its generation of oxidation products such as reactive oxygen species (ROS). At low nontoxic concentrations (in general < 50 mu M), treatment with resveratrol increased viability in a set of representative cell models, whereas application of quenchers of ROS completely truncated these beneficial effects. Notably, resveratrol treatment led to mild, Nrf2-specific gene expression reprogramming. For example, in primary epidermal keratinocytes derived from human skin this coordinated process resulted in a 1.3-fold increase of endogenously generated glutathione (GSH) and subsequently in a quantitative reduction of the cellular redox environment by 2.61 mV mmol GSH per g protein. After induction of oxidative stress by using 0.78% (v/v) ethanol, endogenous generation of ROS was consequently reduced by 24% in resveratrol pre-treated cells. In contrast to the common perception that resveratrol acts mainly as a chemical antioxidant or as a target protein-specific ligand, we propose that the cellular response to resveratrol treatment is essentially based on oxidative triggering. In physiological microenvironments this molecular training can lead to hormetic shifting of cellular defense towards a more reductive state to improve physiological resilience to oxidative stress.
KW  - Trans-reservatrol
KW  - Hydrogen-peroxide
KW  - In-vitro
KW  - Hormesis
KW  - Ethanol
KW  - Oxygen
KW  - Nrf2
KW  - Glutathione
KW  - Metabolism
KW  - Polyphenols
KW  - ROS
KW  - Oxidative stress
KW  - Redox environment
KW  - Skin
KW  - Epidermis
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-187186
VL  - 99
ER  - 
TY  - JOUR
A1  - Youssif, Khayrya A.
A1  - Haggag, Eman G.
A1  - Elshamy, Ali M.
A1  - Rabeh, Mohamed A.
A1  - Gabr, Nagwan M.
A1  - Seleem, Amany
A1  - Salem, M. Alaraby
A1  - Hussein, Ahmed S.
A1  - Krischke, Markus
A1  - Mueller, Martin J.
A1  - Ramadan Abdelmohsen, Usama
T1  - Anti-Alzheimer potential, metabolomic profiling and molecular docking of green synthesized silver nanoparticles of Lampranthus coccineus and Malephora lutea aqueous extracts
JF  - PLoS ONE
N2  - The green synthesis of silver nanoparticles (SNPs) using plant extracts is an eco-friendly method. It is a single step and offers several advantages such as time reducing, cost-effective and environmental non-toxic. Silver nanoparticles are a type of Noble metal nanoparticles and it has tremendous applications in the field of diagnostics, therapeutics, antimicrobial activity, anticancer and neurodegenerative diseases. In the present work, the aqueous extracts of aerial parts of Lampranthus coccineus and Malephora lutea F. Aizoaceae were successfully used for the synthesis of silver nanoparticles. The formation of silver nanoparticles was early detected by a color change from pale yellow to reddish-brown color and was further confirmed by transmission electron microscope (TEM), UV–visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and energy-dispersive X-ray diffraction (EDX). The TEM analysis of showed spherical nanoparticles with a mean size between 12.86 nm and 28.19 nm and the UV- visible spectroscopy showed λ\(_{max}\) of 417 nm, which confirms the presence of nanoparticles. The neuroprotective potential of SNPs was evaluated by assessing the antioxidant and cholinesterase inhibitory activity. Metabolomic profiling was performed on methanolic extracts of L. coccineus and M. lutea and resulted in the identification of 12 compounds, then docking was performed to investigate the possible interaction between the identified compounds and human acetylcholinesterase, butyrylcholinesterase, and glutathione transferase receptor, which are associated with the progress of Alzheimer’s disease. Overall our SNPs highlighted its promising potential in terms of anticholinesterase and antioxidant activity as plant-based anti-Alzheimer drug and against oxidative stress.
KW  - Nanoparticles
KW  - Silver
KW  - Alzheimer's disease
KW  - Glutathione
KW  - Antioxidants
KW  - Serine proteases
KW  - Brain diseases
KW  - Metabolomics
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202696
VL  - 14
IS  - 11
ER  -