TY - JOUR
A1 - Christl, Manfred
A1 - Türk, M.
A1 - Peters, K.
A1 - Peters, E.-M.
A1 - Schnering, H. G. von
T1 - Octahydro-1,2,3:4,5,6-dimethenopentalen-2-carbonitril, das erste Derivat eines noch unbekannten (CH)\(_{10}\)-Kohlenwasserstoffs
N2 - No abstract available
KW - Organische Chemie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58728
ER -
TY - JOUR
A1 - Schliephake, Andreas W.
A1 - Rethwilm, Axel
T1 - Nuclear Localization of Foamy Virus Gag Precursor Protein
N2 - All foamy viruses give rise to a strong nuclear staining when infected cells are reacted with sera from infected hosts. This nuclear ftuorescence distinguishes foamy viruses from all other retroviruses. The experiments reported here indicate that the foamy virus Gag precursor protein is transiently located in the nuclei of infected cells and this is the likely reason for the typical foamy virus nuclear fluorescence. By using the vaccinia virus expression system, a conserved basic sequence motif in the nucleocapsid domain of foamy virus Cag proteins was identified to be responsible for the nuclear transport of the gag precursor molecule. Tbis motif was also found to be able to direct a heterologous protein, the Gag protein of human immunodeficiency virus, into the nucleus.
KW - Virologie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61371
ER -
TY - JOUR
A1 - Hahn, Heidi
A1 - Baunach, Gerald
A1 - Bräutigam, Sandra
A1 - Mergia, Ayalew
A1 - Neumann-Haefelin, Dieter
A1 - Daniel, Muthiah D.
A1 - McClure, Myra O.
A1 - Rethwilm, Axel
T1 - Reactivity of primate sera to foamy virus Gag and Bet proteins
N2 - In order to establish criteria for the Serodiagnosis of foamy virus infections we investigated the extent to which sera from iofected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M\(_r\) and the cytoplasmic 60K M\(_r\) Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rahbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and Mrican green monkey origin. This was reßected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the Bet protein was restricted to viruses from man and the great apes. Recombinant Gag and Bet proteins expressed in prokaryotes or in insect cells were readily recognized by foamy virus-positive primate sera. Screening serum samples from chimpanzees with HFV Gag and Bet proteins expressed by recombinant baculoviruses revealed that 18 out of 35 (52%) were positive for Gag antibodies. Of these, 13 (72 o/o) showed antiborlies against the Bet protein, indicating that Bet antigen is of value in sero1ogical screening for foamy virus infections.
KW - Virologie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61366
ER -
TY - JOUR
A1 - Siwka, Wieslaw
A1 - Schwinn, Andreas
A1 - Baczko, Knut
A1 - Pardowitz, Iancu
A1 - Mhalu, Fred
A1 - Shao, John
A1 - Rethwilm, Axel
A1 - ter Meulen, Volker
T1 - vpu and env sequence variability of HIV-1 isolates from Tanzania
N2 - No abstract available
KW - Virologie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61355
ER -
TY - JOUR
A1 - Christl, Manfred
A1 - Braun, Martin
A1 - Wolz, E.
A1 - Wagner, W.
T1 - Cycloallene, 9 - 1-Phenyl-1-aza-3,4-cyclohexadien, das erste Isodihydropyridin: Erzeugung und Abfangreaktionen
T1 - Cycloallenes, 9 - 1-Phenyl-1-aza-3,4-cyclohexadiene, the First Isodihydropyridine: Generation and Interception
N2 - No abstract available
KW - Organische Chemie
KW - Isoquinolines
KW - hexahydro-
KW - Cyclobuta[c}pyridines
KW - hexahydro-
KW - Cycloadditions
KW - [2 + 2]- and [4 + 2]-
KW - 3-Azabicyclo{3
KW - 1
KW - 0]hexane
KW - 6
KW - 6-dibromo-3-phenyl-
KW - 2
KW - 4-Pentadienylamine
KW - 3-n-butyl-N-phenyl-
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58714
ER -
TY - JOUR
A1 - Gerstner, E.
A1 - Kemmer, R.
A1 - Christl, Manfred
T1 - Elektrophile Additionen an das Bicyclo[1.1.0]butan-System von Tricyclo[4.1.0.0\(^{2,7}\)]-heptan-Derivaten: Halogen-Elektrophile
T1 - Electrophilic Additions to the Bicyclo[l.l.O)butane System of Tricyclo[4.1.0.0\(^{2,7}\)]heptane Derivatives: Halogen Electrophiles
N2 - No abstract available
KW - Organische Chemie
KW - Norpinanes
KW - preparation
KW - Carbocations
KW - classical and nonclassical
KW - Neighbouring group participation
KW - Halonium ions
KW - Migratory aptitudes in carbocations
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58700
ER -
TY - JOUR
A1 - Christl, Manfred
A1 - Gerstner, E.
A1 - Kemmer, R.
A1 - Llewellyn, G.
A1 - Bentley, T. W.
T1 - Elektrophile Additionen an das Bicyclo[1.1.0]butan-System von 1-Phenyl- und 1-(4-Anisyl)tricyclo[4.1.0.0\(^{2,7}\)]heptan: Säure-katalysierte Reaktionen mit Wasser und Methanol, Anlagerung von Essigsäure und Oxymercurierung
N2 - No abstract available
KW - Organische Chemie
KW - 6-Norpinanols
KW - 6-aryl-
KW - preparation
KW - 6-Norpinyl 3
KW - 5-dinitrobenzoates
KW - hydrolysis
KW - Carbocations
KW - generation and rearrangement
KW - 2-Norcaranols
KW - 1-aryl-
KW - Cyclohept-3-en-1-ols
KW - 3-aryl-
KW - conformation
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58696
ER -
TY - JOUR
A1 - Strik, Werner K.
A1 - Dierks, Thomas
A1 - Franzek, Ernst
A1 - Stöber, Gerald
A1 - Maurer, Konrad
T1 - P 300 asymmetries in schizophrenia revisited with reference-independent methods
N2 - Evidence of hemispheric asymmetries in schizophrenia has been reported from different research areas. Asymmetries in evoked potential P300 topography are still controversial because of inconsistent findings. In the present study. previous results of abnormal lateralization of P300 were replicated in stabilized residual Schizophrenie patients. Auditory P300 was recorded during an odd ball task in which subjeets detected rare target stimuli. Schizophrenie patients had the P300 peak shifted to the right hemisphere and differed signifieantly from age- and sex-matched normal control subjects who had left-lateralized P300 peaks. A comparison of different methods of assessment and analysis of the topographical features of the P300 electric fields showed that the extraction of reference-independent descriptors of P300 topography is a reliable and sensitive method for statistical handling of the maps. The results suggest left hemispheric dysfunction during cognitive tasks in a subgroup of Schizophrenie patients. Inconsistencies between previous sturlies are likely to be due to heterogeneous patient groups, which may have included patients in an acute Schizophrenie episode or patients in clinical remission. lnvestigation of the clinical meaning of P300 alterations requires careful psychopathological definition of the patient groups.
KW - Schizophrenie
KW - Laterality
KW - evoked potentials
KW - electroeneephalography
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63372
ER -
TY - JOUR
A1 - Lesch, K. P.
A1 - Stöber, Gerald
A1 - Balling, U.
A1 - Franzek, Ernst
A1 - Li, S. H.
A1 - Ross, C. A.
A1 - Newman, M.
A1 - Beckmann, H.
A1 - Riederer, P.
T1 - Triplet repeats in clinical subtypes of schizophrenia: variation at the DRPLA (B37 CAG repeat) locus is not associated with periodic catatonia
N2 - Clinical evidence for a dominant mode of inheritance and anticipation in periodic catatonia, a distinct subtype of schizophrenia, indicates that genes with triplet repeat expansions or other unstable repetitive elements affecting gene expression may be involved in the etiology of this disorder. Because patients affected with dentatorubral-pallidoluysian atrophy (DRPLA) may present with "schizophrenic" symptoms, we have investigated the DRPLA (B 37 CAG repeat) locus on chromosome 12 in 41 patients with periodic catatonia. The B 37 CAG repeat locus was highly polymorphic but all alleles in both the patient and control group had repeat sizes within the normal range. We conclude that variation at the DRPLA locus is unlikely to be associated with periodic catatonia. The evidence for dominant inheritance and anticipation as well as the high prevalence of human brain genes containing trinucleotide repeats justifies further screening for triplet repeat expansions in periodic catatonia.
KW - Schizophrenie
KW - Association study
KW - B 37 CAG repeat locus
KW - chromosome 12
KW - schizophrenia
KW - periodic catatonia
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63369
ER -
TY - JOUR
A1 - Strik, Werner K.
A1 - Dierks, Thomas
A1 - Franzek, Ernst
A1 - Stöber, Gerald
A1 - Maurer, Konrad
T1 - P300 in Schizophrenia: Interactions between Amplitudes and Topography
N2 - Low P300 amplitudes and topographical asymmetries have been reponed in schizophrenic patients, but reference-independent amplitude assessment failed to replicate reduced amplitudes. P300 amplitude is conventially assessed at midline electrodes (PZ), anti asymmetric topography as reported in schizophrenics, may conj'ound this measurement. We lnvestigated the possible Interaction between P300 ropography and assessments of amplitudes. ln 41 clinically stable schizophrenics and 31 normal controls, the generalfinding ofreduced amplitudes at the P'l electrode and topographical asymmetrles in the patient group were replicated. ln both groups, a.symmetries of the P300 field (lateralized peaks) reduced the standard amplitude assessment at the midline parletal electrode, but did not Qjfoct the reference-independent, global amplitude assessment. This shows thal asymmetry per se does not imply reduced field strength. in addition, in schizophreraics. but not in controls, there was a significcmt effect oftlae direction of asymmetry on both amplltude measures, amplitudes belng lower with increasing shift ofthe P300 peak to the right side. Considering also the slightly left-lateralized peaks in the normal controls. this suggests rhat only right lateralized P300 peaks upressfunctional deficits in schizophrenics, whereas left lateralized pealcs fall wlthin the physiological variability of the P3OO field. Tht refonnce-independent amplitude assessment is proposed for unambiguous amplitude assessment in order to better define the clinical, psychological and physiopathological mtaning of the P3OO alterations in schizophrenics.
KW - Schizophrenie
KW - Event-related potentials
KW - P300
KW - P300 topography
KW - Brain mappins
KW - Schizophrenia
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63351
ER -
TY - JOUR
A1 - Lampidis, Robert
A1 - Gross, Roy
A1 - Sokolovic, Zeljka
A1 - Goebel, Werner
A1 - Kreft, Jürgen
T1 - The virulence regulator protein of Listeria ivanovii is highly homologous to PrfA from Listeria monocytogenes and both belong to the Crp-Fnr family of transcription regulators
N2 - No abstract available
KW - Biologie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60503
ER -
TY - JOUR
A1 - Sendtner, Michael
A1 - Dittrich, F.
A1 - Hughes, R. A.
A1 - Thoenen, H.
T1 - Actions of CNTF and neurotrophins on degenerating motoneurons : preclinical studies and clinical implications
N2 - Spinal motoneurons innervating skeletal muscle were amongst the first neurons shown to require the presence of their target cells to develop appropriately. Isolated embryonie chick and rat motoneurons have been used to identify neurotrophic factors and cytokines capable of supporting the survival of developing motoneurons. Such factors include ciliary neurotrophic factor (CNTF), which is present physiologically in high amounts in myelinating Schwann cells of peripheral nerves, and brain-derived neurotrophic factor (BDNF) which is synthesized in skeletal muscle and, after peripheral nerve lesion. in Schwann cells. These factors have been further analyzed for their physiological significance in maintaining motoneuron function in vivo, and for their potential therapeutic usefulness in degenerative motoneuron disease. Both CNTF and BDNF are capable of rescuing injured facial motoneurons in newbom rats. Furthermore, CNTF prolongs survival and improves motor function of pmn mice, an animal model for degenerative motoneuron disease, by preventing degeneration of motoneuron axons and somata. Thus treatment of human motoneuron disease with neurotrophic factors should be possible, provided that rational means for application of these factors can be established considering also the appearance of potential side effects.
KW - Neurobiologie
KW - Motor neuron disease; Ciliary neurotrophic factor; Brain-derived neurotrophic factor; Animal models; Neurotrophic factors
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-62939
ER -
TY - JOUR
A1 - Schwarz, Klaus
A1 - Hameister, Horst
A1 - Gessler, Manfred
A1 - Grzeschik, Karl-Heinz
A1 - Hansen-Hagge, Thomas E.
A1 - Bartram, Claus R.
T1 - Confirmation of the localization of the human recombination activating gene 1 (RAG1) to chromosome 11p13
N2 - The human recombination activating gene 1 (RAGl) has previously been mapped to chromosomes 14q and 11 p. Here we confirm the chromosome 11 assignment by two independent approaches: autoradiographic and fluorescence in situ hybridization to metaphase spreads and analysis of human-hamster somatic cell hybrid DNA by the polymerase chain reaction (PCR) and Southern blotting. Our results unequivocally localize RAG1 to llp13.
KW - Biochemie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59136
ER -
TY - JOUR
A1 - Schwartz, Faina
A1 - Neve, Rachel
A1 - Eisenman, Robert
A1 - Gessler, Manfred
A1 - Bruns, Gail
T1 - A WAGR region gene between PAX-6 and FSHB expressed in fetal brain
N2 - Developmental delay or mental retardation is a frequent component of multi-system anomaly syndromes associated with chromosomal deletions. Isolation of genes involved in the mental dysfunction in these disorders should define loci important in brain formation or function. We have identified a highly conserved locus in the distal part of 11 p 13 that is prominently expressed in fetal brain. Minimal expression is observed in a number of other fetal tissues. The gene maps distal to PAX-6 but proximal to the loci for brain-derived neurotrophic factor (BDNF) and the beta subunit of follicle stimulating hormone (FSHB), within a region previously implicated in the mental retardation component of some WAGR syndrome patients. Within fetal brain, the corresponding transcript is prominent in frontal, motor and primary visual cortex as weil as in the caudate-putamen. The characteristics of this gene, including the striking evolutionary conservation at the locus, suggest that the encoded protein may function in brain development.
KW - Biochemie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59125
ER -
TY - JOUR
A1 - Herbert, M. K.
A1 - Holzer, P.
T1 - Nitric oxide mediates the amplification by interleukin-1β of neurogenic vasodilatation in the rat skin
N2 - No abstract available.
KW - Medizin
KW - Afferent nerve stimulation
KW - Capsaicin
KW - Cutaneous hyperemia
KW - lnterleukin-lβ
KW - Neurogenie inflammation
KW - Nitric oxide (NO)
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59969
ER -
TY - JOUR
A1 - Heinsen, Helmut
A1 - Henn, R.
A1 - Eisenmenger, W.
A1 - Götz, M.
A1 - Bohl, J.
A1 - Bethke, B.
A1 - Lockermann, U.
A1 - Püschel, K.
T1 - Quantitative investigations on the human entorhinal area: left - right asymmetry and age-related changes
N2 - The total nerve cell numbers in the right and in the left human entorhinal areas have been calculated by volume estimations with the Cavalieri principle and by cell density determinations with the optical disector. Thick gallocyanin-stained serial frozen sections through the parahippocampal gyrus of 22 human subjects (10 female, 12 male) ranging from 18 to 86 years were analysed. The laminar composition of gallocyanin (Nissl)-stained sections could easily be compared with Braak's (1972, 1980) pigmentoarchitectonic study, and Braak's nomenclature of the entorhinal laminas was adopted. Cellsparse laminae dissecantes can more clearly be distinguished in Nissl than in aldehydefuchsin preparations. These cell-poor dissecantes, lamina dissecans extema (dis-ext), lamina dissecans 1 (dis-1) and lamina dissecans 2 (dis-2), were excluded from nerve cell nurober determinations. An exact delineation of the entorhinal area is indispensable for any kind of quantitative investigation. We have defined the entorhinal area by the presence of pre-alpha ceil clusters and the deeper layers of lamina principalis externa (pre-beta and gamma) separated from lamina principalis interna (pri) by lamina dissecans 1 (dis-1). The human entorhinal area is quantitatively characterized by a left-sided (asymmetric) higher pre-alpha cell number and an age-related nerve cell loss in pre as well as pri layers. At variance with other CNS cortical and subcortical structures, the neuronal number of the entorhinal area appears to decrease continuously from the earliest stages analysed, although a secular trend has to be considered. The asymmetry in pre-alpha cell number is discussed in the context of higher human mental capabilities, especially language.
KW - Medizin
KW - Human entorhinal area
KW - Ageing
KW - Lateralitity
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59946
ER -
TY - JOUR
A1 - Gründemann, Dirk
A1 - Gorboulev, Valentin
A1 - Gambaryan, Stepan
A1 - Veyhl, Maike
A1 - Koepsell, Hermann
T1 - Drug excretion mediated by a new prototype of polyspecific transporter
N2 - CATIO~IC drugs of different types and structures (antihistaminics, antiarrhythmics, sedatives, opiates, cytostatics and antibiotics, for example) are excreted in mammals by epithelial cells of the renal proximal tubules and by hepatocytes in the liver1-4. In the proximal tubules, two functionally disparate transport systems are involved which are localized in the basolateral and luminal plasma membrane and are different from the previously identified neuronal monoamine transporters and A TP-dependent multidrug exporting proteins1-3,5-12. Here we report the isolation of a complementary DNA from rat kidney that encodes a 556-amino-acid membrane protein, OCT1, which has the functional characteristics of organic cation uptake over the basolateral membrane of renal proximal tubules and of organic cation uptake into hepatocytes. OCTl is not homologous to any other known protein and is found in kidney, liver and intestine. As OCTl translocates hydrophobic and hydrophilic organic cations of different structures, it is considered to be a new prolotype of polyspecific transporters that are important for drug elimination.
KW - Biologie
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59327
ER -
TY - JOUR
A1 - Wehgartner, Irma
T1 - Röntgenstrahlen und Archäologie
N2 - Naturwissenschaftliche Untersuchungsmethoden werden heute ganz selbstverständlich zur wissenschaftlichen Erforschung archäologischer Objekte herangezogen. Dies gilt für die Materialbestimmung und die Klärung von Herstellungstechniken ebenso wie für die Feststellung von Alter oder Zugehörigkeit zu einer bestimmten Kulturlandschaft. Zugleich spielen diese Methoden bei der Echtheitsprüfung von Stücken unklarer Provenienz eine nicht unerhebliche Rolle.
KW - Archäologie
KW - Röntgenstrahlung
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-53827
ER -
TY - JOUR
A1 - Heinsen, Helmut
A1 - Strik, M.
A1 - Luther, K.
A1 - Ulmar, G.
A1 - Gangnus, D.
A1 - Jungkunz, G.
A1 - Eisenmenger, W.
A1 - Götz, M.
A1 - Bauer, M.
T1 - Cortical and striatal neurone number in Huntington's disease
N2 - The total cortical and striatal neurone and glial numbers were estimated in five cases of Huntington's disease (three males, two females) and five ageand sex-matched control cases. Serial 500-l-lm-thick gallocyanin-stained frontal sections through the left hemisphere were analysed using Cavalieri's principle for volume and the optical disector for cell density estimations. The average cortical neurone number of five controls (mean age 53±13 years, range 36-72 years) was 5.97x 109±320x 106 , the average number of small striatal neurones was 82 X 106± 15.8 X 106• The left striatum (caudatum, putamen, and accumbens) contained a mean of 273 X 106±53 X 106 glial cells (oligodendrocytes, astrocytes and unc1assifiable glial profiles). The mean cortical neurone number in Huntington's disease patients (mean age 49±14 years, range 36-75 years) was diminished by about 33 % to 3.99x109±218x106 nerve cells (P ::;:::: 0.012, MannWhitney V-test). The mean number of small striatal neurones decreased tremendously to 9.72 X 106 ± 3.64 X 106 (-88 % ). The decrease in total glial cells was less pronounced (193 X 106±26 X 106) but the mean glial index, the numerical ratio of glial cells per neurone, increased from 3.35 to 22.59 in Huntington's disease. Qualitatively, neuronal loss was most pronounced in supragranular layers of primary sensory areas (Brodmann's areae 3,1,2; area 17, area 41). Layer HIc pyramidal cells were preferentially lost in association areas of the temporal, frontal, and parietal lobes, whereas spared layer IV granule cells formed a conspicuous band between layer IH and V in these fields. Methodological issues are discussed in context with previous investigations and similarities and differences of laminar and lobar nerve cellloss in Huntington's disease are compared with nerve cell degent-ration in other neuropsychiatric diseases.
KW - Medizin
KW - Huntington's disease . Human cerebral cortex
KW - Striatum
KW - Neurone number
KW - Stereology
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-55217
ER -
TY - JOUR
A1 - Kleinhans, Martha
T1 - "Schlafende Seel', erinn're dich ..." : Zur Funktion spanischer Literatur in Anna Maria Orteses Roman L'Iguana
N2 - No abstract available
KW - Romanistik
Y1 - 1994
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-54478
ER -