TY  - JOUR
A1  - Horder, Hannes
A1  - Guaza Lasheras, Mar
A1  - Grummel, Nadine
A1  - Nadernezhad, Ali
A1  - Herbig, Johannes
A1  - Ergün, Süleyman
A1  - Teßmar, Jörg
A1  - Groll, Jürgen
A1  - Fabry, Ben
A1  - Bauer-Kreisel, Petra
A1  - Blunk, Torsten
T1  - Bioprinting and differentiation of adipose-derived stromal cell spheroids for a 3D breast cancer-adipose tissue model
JF  - Cells
N2  - Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such as in cancer research. In breast cancer, adipose tissue has been acknowledged as an important part of the tumor microenvironment favoring tumor progression. Therefore, in this study, a 3D-printed breast cancer model for facilitating investigations into cancer cell-adipocyte interaction was developed. First, we focused on the printability of human adipose-derived stromal cell (ASC) spheroids in an extrusion-based bioprinting setup and the adipogenic differentiation within printed spheroids into adipose microtissues. The printing process was optimized in terms of spheroid viability and homogeneous spheroid distribution in a hyaluronic acid-based bioink. Adipogenic differentiation after printing was demonstrated by lipid accumulation, expression of adipogenic marker genes, and an adipogenic ECM profile. Subsequently, a breast cancer cell (MDA-MB-231) compartment was printed onto the adipose tissue constructs. After nine days of co-culture, we observed a cancer cell-induced reduction of the lipid content and a remodeling of the ECM within the adipose tissues, with increased fibronectin, collagen I and collagen VI expression. Together, our data demonstrate that 3D-printed breast cancer-adipose tissue models can recapitulate important aspects of the complex cell–cell and cell–matrix interplay within the tumor-stroma microenvironment
KW  - adipose-derived stromal cells
KW  - adipose tissue
KW  - bioprinting
KW  - breast cancer model
KW  - extracellular matrix
KW  - hyaluronic acid
KW  - spheroids
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-236496
VL  - 10
IS  - 4
ER  - 
TY  - JOUR
A1  - Nadernezhad, Ali
A1  - Ryma, Matthias
A1  - Genç, Hatice
A1  - Cicha, Iwona
A1  - Jüngst, Thomasz
A1  - Groll, Jürgen
T1  - Melt electrowriting of isomalt for high‐resolution templating of embedded microchannels
JF  - Advanced Material Technologies
N2  - Fabrication of microchannels using 3D printing of sugars as fugitive material is explored in different fields, including microfluidics. However, establishing reproducible methods for the controlled production of sugar structures with sub-100 μm dimensions remains a challenge. 

This study pioneers the processing of sugars by melt electrowriting (MEW) enabling the fabrication of structures with so far unprecedented resolution from Isomalt. Based on a systematic variation of process parameters, fibers with diameters down to 20 μm can be fabricated. The flexibility in the adjustment of fiber diameter by on-demand alteration of MEW parameters enables generating constructs with perfusable channels within polydimethylsiloxane molds. These channels have a diameter that can be adjusted from 30 to 200 μm in a single design. 

Taken together, the experiments show that MEW strongly benefits from the thermal and physical stability of Isomalt, providing a robust platform for the fabrication of small-diameter embedded microchannel systems.
KW  - medicine
KW  - sugar glass printing
KW  - embedded  templating
KW  - melt  electrowriting
KW  - microfibers
KW  - microfluidics
KW  - sacrificial printing
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-256401
VL  - 6
IS  - 8
ER  -