TY  - JOUR
A1  - Adolfi, Mateus C.
A1  - Du, Kang
A1  - Kneitz, Susanne
A1  - Cabau, Cédric
A1  - Zahm, Margot
A1  - Klopp, Christophe
A1  - Feron, Romain
A1  - Paixão, Rômulo V.
A1  - Varela, Eduardo S.
A1  - de Almeida, Fernanda L.
A1  - de Oliveira, Marcos A.
A1  - Nóbrega, Rafael H.
A1  - Lopez-Roques, Céline
A1  - Iampietro, Carole
A1  - Lluch, Jérôme
A1  - Kloas, Werner
A1  - Wuertz, Sven
A1  - Schaefer, Fabian
A1  - Stöck, Matthias
A1  - Guiguen, Yann
A1  - Schartl, Manfred
T1  - A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas)
JF  - Scientific Reports
N2  - Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF beta signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes.
KW  - evolutionary genetics
KW  - genetic markers
KW  - genome
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-265672
VL  - 11
IS  - 1
ER  - 
TY  - JOUR
A1  - Hurd, Paul J.
A1  - Grübel, Kornelia
A1  - Wojciechowski, Marek
A1  - Maleszka, Ryszard
A1  - Rössler, Wolfgang
T1  - Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
JF  - Scientific Reports
N2  - In the course of a screen designed to produce antibodies (ABs) with affinity to proteins in the honey bee brain we found an interesting AB that detects a highly specific epitope predominantly in the nuclei of Kenyon cells (KCs). The observed staining pattern is unique, and its unfamiliarity indicates a novel previously unseen nuclear structure that does not colocalize with the cytoskeletal protein f-actin. A single rod-like assembly, 3.7-4.1 mu m long, is present in each nucleus of KCs in adult brains of worker bees and drones with the strongest immuno-labelling found in foraging bees. In brains of young queens, the labelling is more sporadic, and the rod-like structure appears to be shorter (similar to 2.1 mu m). No immunostaining is detectable in worker larvae. In pupal stage 5 during a peak of brain development only some occasional staining was identified. Although the cellular function of this unexpected structure has not been determined, the unusual distinctiveness of the revealed pattern suggests an unknown and potentially important protein assembly. One possibility is that this nuclear assembly is part of the KCs plasticity underlying the brain maturation in adult honey bees. Because no labelling with this AB is detectable in brains of the fly Drosophila melanogaster and the ant Camponotus floridanus, we tentatively named this antibody AmBNSab (Apis mellifera Brain Neurons Specific antibody). Here we report our results to make them accessible to a broader community and invite further research to unravel the biological role of this curious nuclear structure in the honey bee central brain.
KW  - mushroom body calyx
KW  - synaptic complexes
KW  - bodies
KW  - insect
KW  - plasticity
KW  - insights
KW  - genome
KW  - model
KW  - proteins
KW  - methylation
KW  - biological techniques
KW  - cell biology
KW  - developmental biology
KW  - molecular biology
KW  - neuroscience
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-260059
VL  - 11
ER  -