TY - BOOK A1 - Kleinhans, Martha T1 - "Lucidere vault tant a dire comme donnant lumiere" : Untersuchung und Edition der Prosaversionen 2, 4 und 5 des Elucidarium N2 - No abstract available KW - Romanistik Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-54488 N1 - Zugl.: Würzburg, Univ., Diss., 1991 ER - TY - JOUR A1 - Bommakanti, R. K. A1 - Klotz, Karl-Norbert A1 - Dratz, E. A. A1 - Jesaitis, A. J. T1 - A carboxyl-terminal tail peptide of neutrophil chemotactic receptor disrupts its physical complex with G protein N2 - No abstract available KW - Toxikologie Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60456 ER - TY - JOUR A1 - Zillober, Christian T1 - A globally convergent version of the method of moving asymptotes N2 - No abstract available Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31984 ER - TY - JOUR A1 - Herrmann, K. H. A1 - Happ, M. A1 - Möllmann, K.-P. A1 - Tomm, J. W. A1 - Becker, Charles R. A1 - Kraus, M. M. A1 - Yuan, S. A1 - Landwehr, G. T1 - A new model for the absorption coefficient of narrow gap (Hg,Cd)Te that simultaneously considers band tails and band filling N2 - A semiempirical model is presented that correlates the broadening of the absorption edge with both transitions below the energy gap and with transitions by the Kane band model. This model correctly fits both the absorption and luminescence spectra of narrow-gap (Hg,Cd)Te samples that have been grown by the traveling heater method as well as by molecular-beam epitaxy. The accuracy of the band-gap determination is enhanced by this model. Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-37894 ER - TY - JOUR A1 - Staikova, M. A1 - Engels, Bernd A1 - Peric, M. A1 - Peyerimhoff, S. D. T1 - Ab initio calculation of the vibronically averaged hyperfine coupling constants in the two lowest electronic states of H\(_2\)O\(^+\) N2 - No abstract available T2 - Ab initio calculations of the vibronically averaged hyperfine coupling constants in the 1\(^2\)Π\(_u\)(X\(^2\)B\(_1\),A\(^2\)A\(_1\)) state of the water cation KW - Organische Chemie Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58998 ER - TY - JOUR A1 - Kaupp, Martin A1 - Schleyer, Paul von Rague T1 - Ab Initio Study of Structures and Stabilities of Substituted Lead Compounds. Why is Inorganic Lead Chemistry Dominated by Pb\(^{II}\) but Organolead Chemistry by Pb\(^{IV}\)? N2 - No abstract available KW - Anorganische Chemie Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-60069 ER - TY - JOUR A1 - Hock, Robert A1 - Moormann, Antoon A1 - Fischer, Dagmar A1 - Scheer, Ulrich T1 - Absence of somatic histone H1 in oocytes and preblastula embryos of Xenopus laevis N2 - Available data on the occurrence and expression of somatic histone HI during oogenesis and early embryogenesis of Xenopus laevis are contradictory. In particular the reported presence of a large storage pool of histone HIA in oocytes is difficult to reconcile with the high transcriptional activity of all gene classes in this specific cell type. In the present study we have used polyclonal antibodies raised against somatic Xenopus histone HI (HIA and HIA/B) for combined immunoblotting experiments to quantitate HI pools and immunolocalization studies to visualize chromosome- bound HI. Both approaches failed to detect soluble or chromosomal histone HI in vitellogenic oocytes, eggs, and cleavage-stage embryos up to early blastula. In addition, chromatin assembled in Xenopus egg extract was also negative for histone HI as revealed by immunofluorescence microscopy. Lampbrush chromosomes not only lacked histone HI but also the previously identified histone HI-like B4 protein (Smith et al., 1988, Genes Dev. 2,1284-1295). In contrast, chromosomes of eggs and early embryos fluoresced brightly with anti-B4 antibodies. Our results lend further support to the view that histone HI expression is developmentally regulated during Xenopus oogenesis and embryogenesis similar to what is known from other species. Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-41350 ER - TY - JOUR A1 - Krüger, Hans-Peter A1 - Schöch, Heinz T1 - Absenkung der Promillegrenze. Ein zweifelhafter Beitrag zur Verkehrssicherheit N2 - No abstract available Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-41911 ER - TY - CHAP A1 - Schneider, Wolfgang T1 - Acquiring expertise: determinants of exceptional performance N2 - No abstract available. KW - Hochbegabung Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86652 ER - TY - JOUR A1 - Cavari, Benzion A1 - Hong, Yunhan A1 - Funkenstein, Bruria A1 - Moav, Boaz A1 - Schartl, Manfred T1 - All-fish gene constructs for growth hormone gene transfer in fish N2 - In order to develop all-fish expression vectors for microinjection into fertilized fish eggs, we have prepared the following cunstructs: rainbow trout metallothionein a/b and the gilthead seabream growth hormone cDNA (ptMTa-gbsGHcDNA, ptMTb-gsbGHcDNA), carp ß-actin gilthead seabream GH cDNA (pcAßgsbGHcDNA). The inducible metallothionein promoters a and b were cloned from rainbow trout, and the constitutive promoter ß-actin was isolated from carp. The metallothionein promoters were cloned by using the PCR technique. The tMTa contains 430 bp, while the tMTb contains 260 bp (Hong et al. 1992). These two promoters were introduced to pGEM-3Z containing the GH cDNA of Sparus aurata to form ptMTa-gsbGH and ptMTb-gsbGH, respectively. The carp cytoplasmic ß-actin gene was chosen as a source for isolating strong constitutive regulatory sequences. One of these regulatory sequences in pUC118 was Iigated to GH cDNA of S. aurata to form the pcAß-gsbGHcDNA. Expression of the constructs containing the metallothionein promoters was tested in fish cell culture and was found tobe induced effectively by zinc. The ptMTa gsb-GH cDNA construct was microinjected into fertilized carp eggs, and integration in the genome of carp was detected in the DNA isolated from fins at the age of two months. KW - Physiologische Chemie KW - growth hormone gene KW - all-fish genes KW - transgenic fish KW - cellline transfection KW - Sparus aurata Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61612 ER - TY - JOUR A1 - Müller, J. G. A1 - Krenn, V. A1 - Schindler, C. A1 - Czub, S. A1 - Stahl-Henning, C. A1 - Coulibaly, C. A1 - Hunsmann, G. A1 - Kneitz, C. A1 - Kerkau, T. A1 - Rethwilm, A. A1 - ter Meulen, V. A1 - Müller-Hermelink, H. K. T1 - Alterations of Thymus Cortical Epithelium and Interdigitating Dendritic Cells but No Increase of Thymocyte Cell Death in the Early Course of Simian Immunodeficiency Virus Infection JF - American Journal of Pathology N2 - The role of the thymus in the pathogenesis of simian acquired immunodeficiency syndrome was investigated in 18 juvenile rhesus monkeys (Macaca mulatta). The thymus was infected from the first week post-SIVmac inoculation, but the amount of virus-positive cells was very low « 1 in 1 04 T cells) as demonstrated by polymerase chain reaction and in situ hybridization. First morphological alteration was a narrowing of the cortex at 12 and 24 wpi. Morphometry revealed no increase of pyknotic T cells but a decrease of the proliferation rate andflow cytometry showed a reduction of the immature \(CD4^+/CD8^+\) double-positive T cells. Ultrastructural analysis revealed vacuolization, shrinkage, andfinally cytolysis of the cortical epithelial cells and the interdigitating dendritic cells. Immunofluorescence staining exhibited a widespread loss of cortical epithelial cells. This damage to the thymic microenvironment could explain the breakdown of the intrathymic T cell proliferation. It preceded fully developed simian acquired immunodeficiency syndrome and is therefore considered to play a major role in its pathogenesis. Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-128250 VL - 143 IS - 3 ER - TY - JOUR A1 - Müller, J. G. A1 - Krenn, V. A1 - Schindler, C. A1 - Czub, S. A1 - Stahl-Henning, C. A1 - Coulibaly, C. A1 - Hunsmann, G. A1 - Kneitz, C. A1 - Kerkau, Thomas A1 - Rethwilm, Axel A1 - terMeulen, Volker T1 - Alterations of thymus cortical epithelium and interdigitating dendritic cells but no increase of thymocyte cell death in the early course of simian immunodeficiency virus infection N2 - No abstract available Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-32583 ER - TY - JOUR A1 - Kredler, Christian A1 - Zillober, Christian A1 - Johannes, Frank A1 - Sigl, Georg T1 - An application of preconditioned conjugate gradients to relative placement in chip design N2 - In distance geometry problems and many other applications, we are faced with the optimization of high-dimensional quadratic functions subject to linear equality constraints. A new approach is presented that projects the constraints, preserving sparsity properties of the original quadratic form such that well-known preconditioning techniques for the conjugate gradient method remain applicable. Very-largescale cell placement problems in chip design have been solved successfully with diagonal and incomplete Cholesky preconditioning. Numerical results produced by a FORTRAN 77 program illustrate the good behaviour of the algorithm. Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31996 ER - TY - CHAP A1 - Cantoreggi, S. A1 - Gupta, R. C. A1 - Lutz, Werner K. T1 - An improved 32P-postlabelling assay for detection and quantitation of styrene 7,8-oxide-DNA adducts N2 - Using DNA modified with [7-3H]styrene 7,8-oxide (SO) in vitro we have standardized the 32P-postlabelling assay for detecting SO-DNA adducts. Nuclease P 1-enriched adducts were 32P-labelled and purified by high-salt ( 4.0 M ammonium formate, pH 6.1} C1s reverse-phase TLC. After elution from the layer with 2-butoxyethanol:H20 (4:6), adducts were separated by two-dimensional PEI cellulose TLC in non-urea solvents (2.0 M ammonium formate, pH 3.5, and 2.7 M sodium phosphate, pH 5.6). One major, three minor and several trace adducts were detected. The efficiency of the kinase reaction depended on the ATP concentration. Use of standard labelling conditions (['Y· 32P]ATP, <3000 Ci/mmol; <2 Mikromol) resulted in poor ( 4-7%) adduct recovery. An ATP concentration of 40 Mikromol, however, increased the labeJling efficiency by a factor of 5-8 (35-55% based on 3H-SO labelied DNA). The results indicate that the new separation technique is suitable for the relatively polar SO-DNA adducts and that high labelling efficiency can be achieved. KW - Medizin Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86305 ER - TY - JOUR A1 - Stopper, Helga A1 - Körber, C. A1 - Spencer, D. L. A1 - Kirchner, S. A1 - Caspary, W.J. A1 - Schiffmann, D. T1 - An investigation of micronucleus and mutation induction by oxazepam in mammalian cells N2 - Tbe benzodiazepines are a class of d.rugs that are widely used in the treatment of various psychiatric disorders. One member of um ~' oxazepam, is also a common metabolite of sevmd other benzod.iazepines. Since the evidence for the genetic toxicity and carcinogenic properties of these compounds is incol:lsb1ent, we investigated the oxazepam-induced fonnation of micronuclei in Syrian Hamster embryo fibroblast (SHE) cells, human amniotic fluid fibroblast-like (AFFL) cells and LS178Y mouse cells. A dose-dependent increase in micronucleus fractions was found in all tbree ceU llnes. The time course of micronucleus induction in L5178Y cells showed a maximum at 5 h after treatment, suggesting that the micronuclei were fonned in the first mitosis after treatment. Kinetochore staining (CREST -antiserum) revealed the presence of kinetochores in -SO% of the micronuclei in aU tbree ceU types. ThJs resu1t was further confinned by in situ bybridization in LS178Y cells and indicates tbe presence of wbole Chromosomes or centric fragments as weU as acentric fragments in the oxazepam-induced micronuclei. The LS178Y cells did not show a mutagenic response to oxazepam at any of the doses or expression times used. KW - Toxikologie Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-63404 ER - TY - JOUR A1 - Gittins, C. M. A1 - Harris, N. A. A1 - Field, R. W. A1 - Verges, J. A1 - Ernst, W. E. A1 - Bündgen, P. A1 - Engels, Bernd T1 - Analysis and Depertubation of the C\(^2\)Π and D\(^2\)Σ\(^+\) states of CaF N2 - No abstract available KW - Organische Chemie Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-58980 ER - TY - JOUR A1 - Hacker, Jörg A1 - Ott, Manfred A1 - Wintermeyer, Eva A1 - Ludwig, Birgit A1 - Fischer, Gunter T1 - Analysis of virulence factors of Legionella pneumophila. N2 - Legionella pneumophila, the causative agent of Legionnaires' disease is a facultative intracellular bacterium, which in the course of human infection multiplies in lung macrophages predominantly manifesting as pneumonia. The natural habitat of Legionella is found in sweet water reservoirs and man-made water systems. Virulent L. pneumophila spontaneously convert to an avirulent status at a high frequency. Genetic approaches have led to the identification of various L. pneumophila genes. The mip (macrophage infectivity potentiator) determinant remains at present the sole established virulence factor. The Mip protein exhibits activity of a peptidyl prolyl cis trans isomerase (PPiase), an enzyme which is able to bind the immunosuppressant FK506 and is involved in protein folding. The recently cloned major outer membrane protein (MOMP) could play a role in the uptake of legionellae by macrophages. Cellular models are useful in studying the intracellular replication of legionellae in eukaryotic cells. Human celllines and protozoan models are appropriate for this purpose. By using U 937 macrophage-like cells and Acanthamoeba castellanii as hosts, we could discriminate virulent and avirulent L. pneumophila variants since only the virulent strain was capable of intracellular growth at 37 oc. By using these systems we further demonstrated that a hemolytic factor cloned and characterized in our laboratory, legiolysin (lly), had no influence on the intracellular growth of L. pneumophila. KW - Legionella pneumophila Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-70620 ER - TY - CHAP A1 - Kreuzer, H. A1 - Henjes-Kunst, F. A1 - Seidel, E. A1 - Schüssler, Ulrich A1 - Bühn, B. T1 - Ar-Ar spectra on minerals from KTB and related medium-pressure units N2 - No abstract available. KW - Kontinentales Tiefbohrprogramm Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86731 ER - TY - JOUR A1 - Falk, Michael A1 - Marohn, Frank T1 - Asymptotically optimal tests for conditional distributions N2 - No abstract available Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45823 ER - TY - CHAP A1 - Hoffmann, Joachim T1 - Aufmerksamkeit, Automatisierung und antizipative Verhaltensteuerung N2 - no abstract available KW - Automation KW - Sportmotorik KW - Kongress KW - Saarbrücken <1992> KW - Techniktraining KW - Bewegungssteuerung KW - Automatismus KW - Aufmerksamkeit Y1 - 1993 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78315 ER -