TY  - JOUR
A1  - Bassler, Miriam C.
A1  - Knoblich, Mona
A1  - Gerhard-Hartmann, Elena
A1  - Mukherjee, Ashutosh
A1  - Youssef, Almoatazbellah
A1  - Hagen, Rudolf
A1  - Haug, Lukas
A1  - Goncalves, Miguel
A1  - Scherzad, Agmal
A1  - Stöth, Manuel
A1  - Ostertag, Edwin
A1  - Steinke, Maria
A1  - Brecht, Marc
A1  - Hackenberg, Stephan
A1  - Meyer, Till Jasper
T1  - Differentiation of salivary gland and salivary gland tumor tissue via Raman imaging combined with multivariate data analysis
JF  - Diagnostics
N2  - Salivary gland tumors (SGTs) are a relevant, highly diverse subgroup of head and neck tumors whose entity determination can be difficult. Confocal Raman imaging in combination with multivariate data analysis may possibly support their correct classification. For the analysis of the translational potential of Raman imaging in SGT determination, a multi-stage evaluation process is necessary. By measuring a sample set of Warthin tumor, pleomorphic adenoma and non-tumor salivary gland tissue, Raman data were obtained and a thorough Raman band analysis was performed. This evaluation revealed highly overlapping Raman patterns with only minor spectral differences. Consequently, a principal component analysis (PCA) was calculated and further combined with a discriminant analysis (DA) to enable the best possible distinction. The PCA-DA model was characterized by accuracy, sensitivity, selectivity and precision values above 90% and validated by predicting model-unknown Raman spectra, of which 93% were classified correctly. Thus, we state our PCA-DA to be suitable for parotid tumor and non-salivary salivary gland tissue discrimination and prediction. For evaluation of the translational potential, further validation steps are necessary.
KW  - salivary gland tumor
KW  - confocal Raman imaging
KW  - principal component analysis
KW  - discriminant analysis
KW  - multivariate data analysis
KW  - molecular diagnostics
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-355558
SN  - 2075-4418
VL  - 14
IS  - 1
ER  - 
TY  - JOUR
A1  - Stefanakis, Mona
A1  - Bassler, Miriam C.
A1  - Walczuch, Tobias R.
A1  - Gerhard-Hartmann, Elena
A1  - Youssef, Almoatazbellah
A1  - Scherzad, Agmal
A1  - Stöth, Manuel Bernd
A1  - Ostertag, Edwin
A1  - Hagen, Rudolf
A1  - Steinke, Maria R.
A1  - Hackenberg, Stephan
A1  - Brecht, Marc
A1  - Meyer, Till Jasper
T1  - The impact of tissue preparation on salivary gland tumors investigated by Fourier-transform infrared microspectroscopy
JF  - Journal of Clinical Medicine
N2  - Due to the wide variety of benign and malignant salivary gland tumors, classification and malignant behavior determination based on histomorphological criteria can be difficult and sometimes impossible. Spectroscopical procedures can acquire molecular biological information without destroying the tissue within the measurement processes. Since several tissue preparation procedures exist, our study investigated the impact of these preparations on the chemical composition of healthy and tumorous salivary gland tissue by Fourier-transform infrared (FTIR) microspectroscopy. Sequential tissue cross-sections were prepared from native, formalin-fixed and formalin-fixed paraffin-embedded (FFPE) tissue and analyzed. The FFPE cross-sections were dewaxed and remeasured. By using principal component analysis (PCA) combined with a discriminant analysis (DA), robust models for the distinction of sample preparations were built individually for each parotid tissue type. As a result, the PCA-DA model evaluation showed a high similarity between native and formalin-fixed tissues based on their chemical composition. Thus, formalin-fixed tissues are highly representative of the native samples and facilitate a transfer from scientific laboratory analysis into the clinical routine due to their robust nature. Furthermore, the dewaxing of the cross-sections entails the loss of molecular information. Our study successfully demonstrated how FTIR microspectroscopy can be used as a powerful tool within existing clinical workflows.
KW  - formalin
KW  - fixation
KW  - tissue preparation
KW  - salivary gland neoplasia
KW  - FTIR spectroscopy
KW  - principal component analysis
KW  - discriminant analysis
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304887
SN  - 2077-0383
VL  - 12
IS  - 2
ER  -