TY  - JOUR
A1  - Kadari, Asifiqbal
A1  - Lu, Min
A1  - Li, Ming
A1  - Sekaran, Thileepan
A1  - Thummer, Rajkumar P.
A1  - Guyette, Naomi
A1  - Chu, Vi
A1  - Edenhofer, Frank
T1  - Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells
JF  - Stem Cell Research & Therapy
N2  - Integrating viruses represent robust tools for cellular reprogramming; however, the presence of viral transgenes in induced pluripotent stem cells (iPSCs) is deleterious because it holds the risk of insertional mutagenesis leading to malignant transformation. Here, we combine the robustness of lentiviral reprogramming with the efficacy of Cre recombinase protein transduction to derive iPSCs devoid of transgenes. By genome-wide analysis and targeted differentiation towards the cardiomyocyte lineage, we show that transgene-free iPSCs are superior to iPSCs before Cre transduction. Our study provides a simple, rapid and robust protocol for the generation of clinical-grade iPSCs suitable for disease modeling, tissue engineering and cell replacement therapies.
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-120578
SN  - 1757-6512
VL  - 5
IS  - 2
ER  -