TY  - JOUR
A1  - Springer, Jan
A1  - Walther, Grit
A1  - Rickerts, Volker
A1  - Hamprecht, Axel
A1  - Willinger, Birgit
A1  - Teschner, Daniel
A1  - Einsele, Hermann
A1  - Kurzai, Oliver
A1  - Loeffler, Juergen
T1  - Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR
JF  - Journal of Fungi
N2  - The mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus Fusarium we analysed several different clinical specimens detecting Fusarium spp. commonly found in clinical samples in Germany. Also, a large collection of lung fluid samples of haematological patients was analysed (n = 243). In these, two samples (0.8%) were reproducibly positive, but only one could be confirmed by sequencing. For this case of probable invasive fungal disease (IFD) culture was positive for Fusarium species. Here we describe a rapid, probe-based real-time PCR assay to specifically detect DNA from a broad range of Fusarium species and its application to clinically relevant specimens.
KW  - probe-based real-time PCR
KW  - Fusarium
KW  - bronchoalveolar lavage fluid
KW  - fungal molecular diagnostics
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-193111
SN  - 2309-608X
VL  - 5
IS  - 4
ER  -