TY  - JOUR
A1  - Haack, Stephanie
A1  - Baiker, Sarah
A1  - Schlegel, Jan
A1  - Sauer, Markus
A1  - Sparwasser, Tim
A1  - Langenhorst, Daniela
A1  - Beyersdorf, Niklas
T1  - Superagonistic CD28 stimulation induces IFN‐γ release from mouse T helper 1 cells in vitro and in vivo
JF  - European Journal of Immunology
N2  - Like human Th1 cells, mouse Th1 cells also secrete IFN‐γ upon stimulation with a superagonistic anti‐CD28 monoclonal antibody (CD28‐SA). Crosslinking of the CD28‐SA via FcR and CD40‐CD40L interactions greatly increased IFN‐γ release. Our data stress the utility of the mouse as a model organism for immune responses in humans.
KW  - CD28
KW  - Th1 cells
KW  - cytokine release
KW  - interferon γ
KW  - Superagonistic antibody
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-239028
VL  - 51
IS  - 3
SP  - 738
EP  - 741
ER  - 
TY  - JOUR
A1  - Langenhorst, Daniela
A1  - Haack, Stephanie
A1  - Göb, Selina
A1  - Uri, Anna
A1  - Lühder, Fred
A1  - Vanhove, Bernhard
A1  - Hünig, Thomas
A1  - Beyersdorf, Niklas
T1  - CD28 costimulation of T helper 1 cells enhances cytokine release in vivo
JF  - Frontiers in Immunology
N2  - Compared to naive T cells, differentiated T cells are thought to be less dependent on CD28 costimulation for full activation. To revisit the role of CD28 costimulation in mouse T cell recall responses, we adoptively transferred in vitro generated OT-II T helper (Th) 1 cells into C57BL/6 mice (Thy1.2\(^{+}\)) and then either blocked CD28–ligand interactions with Fab fragments of the anti-CD28 monoclonal antibody (mAb) E18 or deleted CD28 expression using inducible CD28 knock-out OT-II mice as T cell donors. After injection of ovalbumin protein in adjuvant into the recipient mice we observed that systemic interferon (IFN)γ release strongly depended on CD28 costimulation of the Th1 cells, while secondary clonal expansion was not reduced in the absence of CD28 costimulation. For human memory CD4\(^{+}\) T cell responses we also noted that cytokine release was reduced upon inhibition of CD28 costimulation. Together, our data highlight the so far underestimated role of CD28 costimulation for the reactivation of fully differentiated CD4\(^{+}\) T cells.
KW  - CD4\(^{+}\) T helper cells
KW  - T helper 1 cells
KW  - antigenic recall
KW  - CD28 costimulation
KW  - cytokine secretion
KW  - mouse
KW  - human
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-176726
VL  - 9
IS  - 1060
ER  -