TY  - JOUR
A1  - Hahn, Heidi
A1  - Baunach, Gerald
A1  - Bräutigam, Sandra
A1  - Mergia, Ayalew
A1  - Neumann-Haefelin, Dieter
A1  - Daniel, Muthiah D.
A1  - McClure, Myra O.
A1  - Rethwilm, Axel
T1  - Reactivity of primate sera to foamy virus Gag and Bet proteins
N2  - In order to establish criteria for the Serodiagnosis of foamy virus infections we investigated the extent to which sera from iofected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M\(_r\) and the cytoplasmic 60K M\(_r\) Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rahbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and Mrican green monkey origin. This was reßected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the Bet protein was restricted to viruses from man and the great apes. Recombinant Gag and Bet proteins expressed in prokaryotes or in insect cells were readily recognized by foamy virus-positive primate sera. Screening serum samples from chimpanzees with HFV Gag and Bet proteins expressed by recombinant baculoviruses revealed that 18 out of 35 (52%) were positive for Gag antibodies. Of these, 13 (72 o/o) showed antiborlies against the Bet protein, indicating that Bet antigen is of value in sero1ogical screening for foamy virus infections.
KW  - Virologie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61366
ER  - 
TY  - JOUR
A1  - Baunach, Gerald
A1  - Maurer, Bernd
A1  - Hahn, Heidi
A1  - Kranz, Manuela
A1  - Rethwilm, Axel
T1  - Functional analysis of human foamy virus accessory reading frames
N2  - No abstract available
KW  - Virologie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-61398
ER  -