TY  - JOUR
A1  - Manukjan, Georgi
A1  - Ripperger, Tim
A1  - Venturini, Letizia
A1  - Stadler, Michael
A1  - Göhring, Gudrun
A1  - Schambach, Axel
A1  - Schlegelberger, Brigitte
A1  - Steinemann, Doris
T1  - GABP is necessary for stem/progenitor cell maintenance and myeloid differentiation in human hematopoiesis and chronic myeloid leukemia
JF  - Stem Cell Research
N2  - Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of murine hematopoietic and leukemic stem cells. Here, we report the consequences of functional impairment of GABP in human hematopoietic and in leukemic stem/progenitor cells. Ectopic overexpression of a dominant-negative acting GABP mutant led to impaired myeloid differentiation of CD34\(^{+}\) hematopoietic stem/progenitor cells obtained from healthy donors. Moreover, drastically reduced clonogenic capacity of leukemic stem/progenitor cells isolated from bone marrow aspirates of chronic myeloid leukemia (CML) patients underlines the importance of GABP on stem/progenitor cell maintenance and confirms the relevance of GABP for human myelopoiesis in healthy and diseased states.
KW  - GABP
KW  - stem cells
KW  - human hematopoiesis
KW  - leukemia
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-168165
VL  - 16
IS  - 3
ER  -