TY  - JOUR
A1  - Schneider-Schaulies, Jürgen
A1  - Knauer, R.
A1  - Hünig, T.
A1  - Schimpl, A.
A1  - Wecker, E.
T1  - Induction of c-onc expression in polyclonally activated mouse lymphocytes
N2  - No abstract available
KW  - Lymphozyt
Y1  - 1984
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-54784
ER  - 
TY  - JOUR
A1  - Schneider-Schaulies, Jürgen
A1  - Hünig, T.
A1  - Schimpl, A.
A1  - Wecker, E.
T1  - Kinetics of cellular oncogene expression in mouse lymphocytes ; I. Expression of c-myc and c-ras Ha in T lymphocytes induced by various mitogens
N2  - Murine spienie T lymphocytes display maximal cellular myc gene (c-myc) expression already 3 h after concanavalin A timulation and sub equent down-regulation before the onset of DNA syntbesis. Stimulation by leucoagglulinin in the prcsence or absence of interleukin 2 Ieads to only low initiaJ Ievels of c-myc-specific RNA which, however, increase later on. A similar pattero of c-myc expression is shown by the Lyt- 2+ T cell subpopulation stimuiated with eilher concanavalin A or leucoagglutinin in the prescncc of interleukin 2. Although eH]thyn1idine incorporation was identical, the leucoagglutinin-stimulated Lyt-2+ T cells werc void of any demon. trable c-mycspeci. fic RNA at 3 h post-stimulation. Thus, the kinetics of c-myc expression in mause T lymphocytes arenot at all uniform, but depend on the mitogen and the subpopulation. [n contrast, lcvel8 of c-rasH•-spccific R A wcre always low at early times, always increased towards tbe onset ofDNA synthesis and down-regulationwas not observed.
KW  - Immunologie
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-54803
ER  - 
TY  - JOUR
A1  - Schneider-Schaulies, Jürgen
A1  - Schimpl, A.
A1  - Wecker, E.
T1  - Kinetics of cellular oncogene expression in mouse lymphocytes II. Regulation of c-fos and c-myc gene expression
N2  - Newly isolated lymphocytes from mousespleensexpress the c-fos oncogene even in the absence of mitogen with maximal mRNA levels 60 min post preparation of single cell suspension, whereas c-myc mRNA Ievels increase only after mitogenic Stimulation with maximal mRNA Ievels 6 h post Stimulation. The half-lives of c-fos mRNA are generally very short; they increase from 14 min (after 30 min of culture) to 70 min (after 2 h of culture). The half-lives of c-myc mRNA decrease from 50 min (at 2 and 6 h post stimulation with concanavalin A) to 12 min (at 48 h post stimulation). The c-fos gene transcription is already tumed on in time-0 lymphocytes 10 min after disruption of the organ structure of the spleens and is down-regulated after 2 h and later. In nuclear run-on experiments with nonstimulated lymphocytes there is already significant transcription of the first exon of c-myc, but almost no elongation of the transcript to exon 2 and 3. In concanavalin A-treated lymphocytes elongation is stimulated about 5-fold within 6 h and returns to background levels at 48 h post Stimulation. · The nuclear run-on analyses of nonactivated lymphocytes showed a signal for RNA complementary to c-myc mRNA detected with a probe specific for the exon 1/intron 1 boundary of c-myc, which disappeared with increasing time of concanavalin A Stimulation. This anti-sense transcription may play a role in regulating the elongation of cmyc transcripts.
KW  - Lymphozyt
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-54823
ER  - 
TY  - JOUR
A1  - Schneider-Schaulies, Jürgen
A1  - Knauer, R.
A1  - Schimpl, A.
A1  - Wecker, E.
T1  - Cellular Oncogenes and lymphocyte activation
N2  - No abstract available
KW  - Lymphozyt
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-54836
ER  - 
TY  - JOUR
A1  - Kerkau, T.
A1  - Gernert, S.
A1  - Kneitz, C.
A1  - Schimpl, A.
T1  - Mechanism of MHC class I downregulation in HIV infected cells
N2  - HIV infection of CD4+ peripheral blood lymphocytes leads to a loss of MHC dass I molecules on the surface of the infected cells as detectable by monodonal antibody staining and flow cytometry. Incubation of the infected cells at 26 oe or treatment at 37 oe with peptides leads to upregulation of MHC dass I to levels equal to those found on uninfected cells cultured und er the same conditions. The data suggest that, after HIV infection, the mechanisms responsible for peptide generation, peptide transport and thus stable association between peptides and MHC dass I molecules are severely affected.
KW  - HIV
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-56849
ER  -