TY  - JOUR
A1  - Riegmann, N.
A1  - Kusters, R.
A1  - Van Veggel, H.
A1  - Bergmans, H.
A1  - Van Bergen en Henegouwen, P.
A1  - Hacker, Jörg
A1  - Van Die, I.
T1  - F1C fimbriae of an uropathogenic Escherichia coli: Genetic and functional organization of the foc gene cluster and identification of minor subunits
N2  - Tbe genetic organization of tbe foc gene duster bas been studied; six genes involved in tbe biogenesis of Fl C fimbriae were identifi.ed.focA encodes tbe major fimbrial subunit, focC encodes a product tbat is indispensable for fimbria formation,focG andjocH encode minor ftmbrial subunits, andfocl encodes a protein wbicb sbows similarities to the subunit protein FocA. Apart from tbe FocA major subunits, purified FlC fimbriae contain at least two minor subunits, FocG and FocH. Minor proteins of similar size were observed in purified S fimbriae. Remarkably, some mutations in tbe foc gene duster result in an altered 6mbrial morpbology, i.e., rigid stubs or long, curly ftmbriae.
KW  - Infektionsbiologie
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59597
ER  - 
TY  - JOUR
A1  - Van Die, I.
A1  - Kramer, C.
A1  - Hacker, Jörg
A1  - Bergmans, H.
A1  - Jongen, W.
A1  - Hoekstra, W.
T1  - Nucleotide sequence of the genes coding for minor fimbrial subunits of the F1C fimbriae of Escherichia coli
N2  - F 1 C fimbriae allow uropathogenic Escherichia coli to adhere to specific epithelial surfaces. This adhesive property is probably due to the presence of minor fimbrial components in F1C fimbriae. The foe gene cluster encoding F1C fimbriae has been cloned, as described previously. Here we present the nucleotide sequence (2081 bp) coding for the F 1 C minor fimbria I subunits. The structural genes code for polypeptides of 175 (FocF), 166 (FocG), and 300 (FocH) amino acids. The deduced amino acids of the F 1 C minor subunits were compared with the reported sequences of the minor subunits of other types of fimbriae. The data show that the Foc minor subunits are highly homologous to the corresponding Sfa proteins, whereas homology to the minor subunits of type 1 and P fimbriae is much lower.
KW  - Pilus
KW  - Escherichia coli
KW  - Adherence
KW  - Urinary tract
KW  - Foc protein
KW  - Minor subunits
KW  - Sequencing
KW  - Homology
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-40353
ER  -