TY  - JOUR
A1  - Weigel, Tobias
A1  - Malkmus, Christoph
A1  - Weigel, Verena
A1  - Wußmann, Maximiliane
A1  - Berger, Constantin
A1  - Brennecke, Julian
A1  - Groeber‐Becker, Florian
A1  - Hansmann, Jan
T1  - Fully Synthetic 3D Fibrous Scaffolds for Stromal Tissues—Replacement of Animal‐Derived Scaffold Materials Demonstrated by Multilayered Skin
JF  - Advanced Materials
N2  - The extracellular matrix (ECM) of soft tissues in vivo has remarkable biological and structural properties. Thereby, the ECM provides mechanical stability while it still can be rearranged via cellular remodeling during tissue maturation or healing processes. However, modern synthetic alternatives fail to provide these key features among basic properties. Synthetic matrices are usually completely degraded or are inert regarding cellular remodeling. Based on a refined electrospinning process, a method is developed to generate synthetic scaffolds with highly porous fibrous structures and enhanced fiber‐to‐fiber distances. Since this approach allows for cell migration, matrix remodeling, and ECM synthesis, the scaffold provides an ideal platform for the generation of soft tissue equivalents. Using this matrix, an electrospun‐based multilayered skin equivalent composed of a stratified epidermis, a dermal compartment, and a subcutis is able to be generated without the use of animal matrix components. The extension of classical dense electrospun scaffolds with high porosities and motile fibers generates a fully synthetic and defined alternative to collagen‐gel‐based tissue models and is a promising system for the construction of tissue equivalents as in vitro models or in vivo implants.
KW  - 3D scaffolds
KW  - electrospinning
KW  - highly porous materials
KW  - multilayered skin
KW  - stromal tissues
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-276403
VL  - 34
IS  - 10
ER  - 
TY  - JOUR
A1  - Tylek, Tina
A1  - Blum, Carina
A1  - Hrynevich, Andrei
A1  - Schlegelmilch, Katrin
A1  - Schilling, Tatjana
A1  - Dalton, Paul D
A1  - Groll, Jürgen
T1  - Precisely defined fiber scaffolds with 40 μm porosity induce elongation driven M2-like polarization of human macrophages
JF  - Biofabrication
N2  - Macrophages are key players of the innate immune system that can roughly be divided into the pro-inflammatory M1 type and the anti-inflammatory, pro-healing M2 type. While a transient initial pro-inflammatory state is helpful, a prolonged inflammation deteriorates a proper healing and subsequent regeneration. One promising strategy to drive macrophage polarization by biomaterials is precise control over biomaterial geometry. For regenerative approaches, it is of particular interest to identify geometrical parameters that direct human macrophage polarization. For this purpose, we advanced melt electrowriting (MEW) towards the fabrication of fibrous scaffolds with box-shaped pores and precise inter-fiber spacing from 100 μm down to only 40 μm. These scaffolds facilitate primary human macrophage elongation accompanied by differentiation towards the M2 type, which was most pronounced for the smallest pore size of 40 μm. These new findings can be important in helping to design new biomaterials with an enhanced positive impact on tissue regeneration.
KW  - cell elongation
KW  - human macrophages
KW  - melt electrowriting (MEW)
KW  - macrophage polarization
KW  - 3D scaffolds
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-254012
VL  - 12
IS  - 2
ER  -