TY  - JOUR
A1  - Prusty, Bhupesh K.
A1  - Gulve, Nitish
A1  - Govind, Sheila
A1  - Krueger, Gerhard R. F.
A1  - Feichtinger, Julia
A1  - Larcombe, Lee
A1  - Aspinall, Richard
A1  - Ablashi, Dharam V.
A1  - Toro, Carla T.
T1  - Active HHV-6 Infection of Cerebellar Purkinje Cells in Mood Disorders
JF  - Frontiers in Microbiology
N2  - Early-life infections and associated neuroinflammation is incriminated in the pathogenesis of various mood disorders. Infection with human roseoloviruses, HHV-6A and HHV-6B, allows viral latency in the central nervous system and other tissues, which can later be activated causing cognitive and behavioral disturbances. Hence, this study was designed to evaluate possible association of HHV-6A and HHV-6B activation with three different groups of psychiatric patients. DNA qPCR, immunofluorescence and FISH studies were carried out in post-mortem posterior cerebellum from 50 cases each of bipolar disorder (BPD), schizophrenia, 15 major depressive disorder (MDD) and 50 appropriate control samples obtained from two well-known brain collections (Stanley Medical Research Institute). HHV-6A and HHV-6B late proteins (indicating active infection) and viral DNA were detected more frequently (p < 0.001 for each virus) in human cerebellum in MDD and BPD relative to controls. These roseolovirus proteins and DNA were found less frequently in schizophrenia cases. Active HHV-6A and HHV-6B infection in cerebellar Purkinje cells were detected frequently in BPD and MDD cases. Furthermore, we found a significant association of HHV-6A infection with reduced Purkinje cell size, suggesting virus-mediated abnormal Purkinje cell function in these disorders. Finally, gene expression analysis of cerebellar tissue revealed changes in pathways reflecting an inflammatory response possibly to HHV-6A infection. Our results provide molecular evidence to support a role for active HHV-6A and HHV-6B infection in BPD and MDD.
KW  - HHV-6
KW  - bipolar disorder
KW  - schizophrenia
KW  - major depressive disorder
KW  - Purkinje cells
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-369222
VL  - 9
ER  - 
TY  - JOUR
A1  - Heinsen, Helmut
A1  - Heinsen, Y. L.
T1  - Quantitative studies on regional differences in Purkinje cell dendritic spines and parallel fiber synaptic density
N2  - No abstact available
KW  - Medizin
KW  - Cerebellar cortex
KW  - Albino rats
KW  - Quantitative anatomy
KW  - Purkinje cells
KW  - Spines
KW  - Parallel fiber synapses
KW  - Regional differences
Y1  - 1983
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59917
ER  - 
TY  - JOUR
A1  - Heinsen, Helmut
T1  - Regional differences in the distribution of lipofuscin in Purkinje cell perikarya : a quantitative Pigmentarchitectonic study of the Cerebellar Cortexof Senile Albino Rats
N2  - The distribution of lipofuscin in the perikarya of Purkin je cells of vermal and hemispheric lobules has been determined quantitatively in 7 rats, 30-38 months old, by the point-counting method. On the basis of morphologically and statistically significant differences a pigmentarchitectonics of the cerebellar cortex is established. The Purkinje cells of lobule VIa (Larsell 1952) are extremely lipofuscin-rich. The Purkinje cells of the hemispheres, lobules V, Vlb + c and VII contain considerable amounts of a finely granular lipofuscin, the Purkinje cells of lobules I-III and VIII- IXa a globular type of lipofuscin. The Purkinje cells of sublobule XI d c and X are lipofuscin-poor cells. Three types of lipofuscin ha ve been identified in the light microscope.
KW  - Medizin
KW  - Lipofuscin
KW  - Purkinje cells
KW  - Pigmentarchitectonics
KW  - Senile rat
Y1  - 1981
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-59904
ER  - 
TY  - JOUR
A1  - Heinsen, Helmut
A1  - Heinsen, Y. L.
T1  - Regionale Unterschiede der numerischen Purkinje-Zell-Dichte in Kleinhirnen von Albinoratten zweier Stämme
N2  - The total number and the number of Purkinje cells per square millimeter of the surface of the cerebellar granular layer were measured in albino rats from two strains: Chbb-THOM-Wist and Han: SPRD. The total number of the Purkinje cells in the cerebellar vermian lobules L land L X with the exception of cells in the lobules VIb + c did not. differ significantly. Statistically significant differences were found between the individuals of the two rat strains and within single lobules of the cerebella concerning the cell density of Purkinje cells per square millimeter surface of the granular layer. The results suggest heterogeneity of the cerebellar cortex between cerebellar lobules and between albino rats derived from different strains.
KW  - Albinoratten
KW  - Purkinje-Zellen
KW  - Quantitative Anatomie
KW  - Albino rats
KW  - Purkinje cells
KW  - Quantitative anatomy
Y1  - 1983
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45752
ER  -