TY  - JOUR
A1  - Rockel, Anna F.
A1  - Wagner, Nicole
A1  - Spenger, Peter
A1  - Ergün, Süleyman
A1  - Wörsdörfer, Philipp
T1  - Neuro-mesodermal assembloids recapitulate aspects of peripheral nervous system development \(in\) \(vitro\)
JF  - Stem Cell Reports
N2  - Summary
Here we describe a novel neuro-mesodermal assembloid model that recapitulates aspects of peripheral nervous system (PNS) development such as neural crest cell (NCC) induction, migration, and sensory as well as sympathetic ganglion formation. The ganglia send projections to the mesodermal as well as neural compartment. Axons in the mesodermal part are associated with Schwann cells. In addition, peripheral ganglia and nerve fibers interact with a co-developing vascular plexus, forming a neurovascular niche. Finally, developing sensory ganglia show response to capsaicin indicating their functionality.

The presented assembloid model could help to uncover mechanisms of human NCC induction, delamination, migration, and PNS development. Moreover, the model could be used for toxicity screenings or drug testing. The co-development of mesodermal and neuroectodermal tissues and a vascular plexus along with a PNS allows us to investigate the crosstalk between neuroectoderm and mesoderm and between peripheral neurons/neuroblasts and endothelial cells.

Highlights
•Novel neuro-mesodermal assembloid model of peripheral nervous system development
•Model covers neural crest cell induction, migration, and ganglion formation
•Ganglia send projections to the mesodermal as well as neural compartment
•Peripheral ganglia and nerve fibers interact with a co-developing vascular plexus
KW  - peripheral nervous system
KW  - neural crest
KW  - sensory ganglia
KW  - sensory neuron
KW  - vasculature
KW  - blood vessel
KW  - neural organoid
KW  - mesodermal organoid
KW  - assembloid
KW  - human induced pluripotent stem cells
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-349925
SN  - 2213-6711
VL  - 18
IS  - 5
ER  - 
TY  - JOUR
A1  - Agoston, Zsuzsa
A1  - Li, Naixin
A1  - Haslinger, Anja
A1  - Wizenmann, Andrea
A1  - Schulte, Dorothea
T1  - Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
JF  - BMC Developmental Biology
N2  - Background: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for the specification and subsequent development of the adjacent mid-and hindbrain. Much less, however, is known about the regulatory relationship and functional interaction of molecules that are expressed in the tectal anlage after tectal fate specification has taken place and tectal development has commenced. 
Results: Here, we provide experimental evidence for reciprocal regulation and subsequent cooperation of the paired-type transcription factors Pax3, Pax7 and the TALE-homeodomain protein Meis2 in the tectal anlage. Using in ovo electroporation of the mesencephalic vesicle of chick embryos we show that (i) Pax3 and Pax7 mutually regulate each other's expression in the mesencephalic vesicle, (ii) Meis2 acts downstream of Pax3/7 and requires balanced expression levels of both proteins, and (iii) Meis2 physically interacts with Pax3 and Pax7. These results extend our previous observation that Meis2 cooperates with Otx2 in tectal development to include Pax3 and Pax7 as Meis2 interacting proteins in the tectal anlage. 
Conclusion: The results described here suggest a model in which interdependent regulatory loops involving Pax3 and Pax7 in the dorsal mesencephalic vesicle modulate Meis2 expression. Physical interaction with Meis2 may then confer tectal specificity to a wide range of otherwise broadly expressed transcriptional regulators, including Otx2, Pax3 and Pax7.
KW  - dosage
KW  - quali-chick chimeras
KW  - drosophila embryo
KW  - neural crest
KW  - transcription activation
KW  - hindbrain boundary
KW  - isthmic oragnizer
KW  - sonic hedghog
KW  - expression
KW  - induction
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-132626
VL  - 12
IS  - 10
ER  -