TY  - JOUR
A1  - Geiger, Nina
A1  - König, Eva-Maria
A1  - Oberwinkler, Heike
A1  - Roll, Valeria
A1  - Diesendorf, Viktoria
A1  - Fähr, Sofie
A1  - Obernolte, Helena
A1  - Sewald, Katherina
A1  - Wronski, Sabine
A1  - Steinke, Maria
A1  - Bodem, Jochen
T1  - Acetylsalicylic acid and salicylic acid inhibit SARS-CoV-2 replication in precision-cut lung slices
JF  - Vaccines
N2  - Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino- and influenza viruses at high concentrations. We sought to investigate whether ASA and its metabolite salicylic acid (SA) inhibit SARS-CoV-2 since it might use similar pathways to influenza viruses. The compound-treated cells were infected with SARS-CoV-2. Viral replication was analysed by RTqPCR. The compounds suppressed SARS-CoV-2 replication in cell culture cells and a patient-near replication system using human precision-cut lung slices by two orders of magnitude. While the compounds did not interfere with viral entry, it led to lower viral RNA expression after 24 h, indicating that post-entry pathways were inhibited by the compounds.
KW  - acetylsalicylic acid
KW  - salicylic acid
KW  - antiviral activity
KW  - aspirin
KW  - SARS-CoV-2
KW  - precision-cut lung slices
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-289885
SN  - 2076-393X
VL  - 10
IS  - 10
ER  - 
TY  - JOUR
A1  - Hyun, Tae Kyung
A1  - van der Graaff, Eric
A1  - Albacete, Alfonso
A1  - Eom, Seung Hee
A1  - Grosskinsky, Dominik K.
A1  - Böhm, Hannah
A1  - Janschek, Ursula
A1  - Rim, Yeonggil
A1  - Ali, Walid Wahid
A1  - Kim, Soo Young
A1  - Roitsch, Thomas
T1  - The Arabidopsis PLAT Domain Protein1 is Critically Involved in Abiotic Stress Tolerance
JF  - PLOS ONE
N2  - Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.
KW  - salicylic acid
KW  - gene expression
KW  - signal transduction
KW  - cold stress
KW  - salt stress
KW  - abscisic acid
KW  - endoplasmatic reticulum
KW  - transcription factors
KW  - pseudomonas syringae
KW  - plants response
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-114648
VL  - 9
IS  - 11
ER  -