TY  - JOUR
A1  - Gergs, Ulrich
A1  - Jahn, Tina
A1  - Schulz, Nico
A1  - Großmann, Claudia
A1  - Rueckschloss, Uwe
A1  - Demus, Uta
A1  - Buchwalow, Igor B.
A1  - Neumann, Joachim
T1  - Protein phosphatase 2A improves cardiac functional response to ischemia and sepsis
JF  - International Journal of Molecular Sciences
N2  - Reversible protein phosphorylation is a posttranslational modification of regulatory proteins involved in cardiac signaling pathways. Here, we focus on the role of protein phosphatase 2A (PP2A) for cardiac gene expression and stress response using a transgenic mouse model with cardiac myocyte-specific overexpression of the catalytic subunit of PP2A (PP2A-TG). Gene and protein expression were assessed under basal conditions by gene chip analysis and Western blotting. Some cardiac genes related to the cell metabolism and to protein phosphorylation such as kinases and phosphatases were altered in PP2A-TG compared to wild type mice (WT). As cardiac stressors, a lipopolysaccharide (LPS)-induced sepsis in vivo and a global cardiac ischemia in vitro (stop-flow isolated perfused heart model) were examined. Whereas the basal cardiac function was reduced in PP2A-TG as studied by echocardiography or as studied in the isolated work-performing heart, the acute LPS- or ischemia-induced cardiac dysfunction deteriorated less in PP2A-TG compared to WT. From the data, we conclude that increased PP2A activity may influence the acute stress tolerance of cardiac myocytes.
KW  - protein phosphorylation
KW  - PP2A
KW  - transgenic mice
KW  - heart
KW  - LPS
KW  - sepsis
KW  - ischemia
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-284035
SN  - 1422-0067
VL  - 23
IS  - 9
ER  -