TY  - THES
A1  - Paul, Jens Christian
T1  - Retrospektive Analyse von 119 Patienten mit kutanen Sarkomen an der Universitäts-Hautklinik Würzburg aus den Jahren 1999-2009: Diagnostik, Therapie und Prognose
T1  - Analysis of Survival, Therapy and progostic factors within a cohort of 119 dermatological patients with cutaneous sarcoma at the Universitätsklinik Würzburg
N2  - In dieser Studie werden demographische, diagnostische und therapeutische Faktoren einer an kutanen Sarkomen erkrankten Patientengruppe der Würzburger Hautklinik untersucht. Der prognostische Wert dieser Faktoren wird mit Hilfe verschiedener statistischer Tests und Vergleiche untersucht. Die Studienpopulation umfasst 119 Patienten, die im Zeitraum von 1999 bis 2009 behandelt wurden.
N2  - Analysis of Survival, Therapy and progostic factors within a cohort of 119 dermatological patients with cutaneous sarcoma at the Universitätsklinik Würzburg
KW  - Kutane Sarkome
KW  - Sarkom
KW  - Klinik
KW  - Prognose
KW  - Therapie
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116846
ER  - 
TY  - JOUR
A1  - Shityakov, Sergey
A1  - Förster, Carola
A1  - Rethwilm, Axel
A1  - Dandekar, Thomas
T1  - Evaluation and Prediction of the HIV-1 Central Polypurine Tract Influence on Foamy Viral Vectors to Transduce Dividing and Growth-Arrested Cells
N2  - Retroviral vectors are potent tools for gene delivery and various biomedical applications. To accomplish a gene transfer task successfully, retroviral vectors must effectively transduce diverse cell cultures at different phases of a cell cycle. However, very promising retroviral vectors based on the foamy viral (FV) backbone lack the capacity to efficiently transduce quiescent cells. It is hypothesized that this phenomenon might be explained as the inability of foamy viruses to form a pre-integration complex (PIC) with nuclear import activity in growth-arrested cells, which is the characteristic for lentiviruses (HIV-1). In this process, the HIV-1 central polypurine tract (cPPT) serves as a primer for plus-strand synthesis to produce a “flap” element and is believed to be crucial for the subsequent double-stranded cDNA formation of all retroviral RNA genomes. In this study, the effects of the lentiviral cPPT element on the FV transduction potential in dividing and growth-arrested (G1/S phase) adenocarcinomic human alveolar basal epithelial (A549) cells are investigated by experimental and theoretical methods. The results indicated that the HIV-1 cPPT element in a foamy viral vector background will lead to a significant reduction of the FV transduction and viral titre in growth-arrested cells due to the absence of PICs with nuclear import activity.
KW  - Evaluation
KW  - Prognose
KW  - HIV
KW  - Spumaviren
KW  - Einfluss
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-112763
ER  -