TY  - JOUR
A1  - Schartl, Manfred
A1  - Kneitz, Susanne
A1  - Volkoff, Helene
A1  - Adolfi, Mateus
A1  - Schmidt, Cornelia
A1  - Fischer, Petra
A1  - Minx, Patrick
A1  - Tomlinson, Chad
A1  - Meyer, Axel
A1  - Warren, Wesley C.
T1  - The piranha genome provides molecular insight associated to its unique feeding behavior
JF  - Genome Biology and Evolution
N2  - The piranha enjoys notoriety due to its infamous predatory behavior but much is still not understood about its evolutionary origins and the underlying molecular mechanisms for its unusual feeding biology. We sequenced and assembled the red-bellied piranha (Pygocentrus nattereri) genome to aid future phenotypic and genetic investigations. The assembled draft genome is similar to other related fishes in repeat composition and gene count. Our evaluation of genes under positive selection suggests candidates for adaptations of piranhas’ feeding behavior in neural functions, behavior, and regulation of energy metabolism. In the fasted brain, we find genes differentially expressed that are involved in lipid metabolism and appetite regulation as well as genes that may control the aggression/boldness behavior of hungry piranhas. Our first analysis of the piranha genome offers new insight and resources for the study of piranha biology and for feeding motivation and starvation in other organisms.
KW  - whole-genome sequencing
KW  - genome annotation
KW  - comparative genomics
KW  - RNA-seq transcriptome
KW  - energy homeostasis
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202218
VL  - 11
IS  - 8
ER  - 
TY  - JOUR
A1  - Herpin, Amaury
A1  - Schmidt, Cornelia
A1  - Kneitz, Susanne
A1  - Gobé, Clara
A1  - Regensburger, Martina
A1  - Le Cam, Aurélie
A1  - Montfort, Jérome
A1  - Adolfi, Mateus C.
A1  - Lillesaar, Christina
A1  - Wilhelm, Dagmar
A1  - Kraeussling, Michael
A1  - Mourot, Brigitte
A1  - Porcon, Béatrice
A1  - Pannetier, Maëlle
A1  - Pailhoux, Eric
A1  - Ettwiller, Laurence
A1  - Dolle, Dirk
A1  - Guiguen, Yann
A1  - Schartl, Manfred
T1  - A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka
JF  - PLoS Biology
N2  - Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1—acting as master sex-determining gene—has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3′ UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans—together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells—suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates.
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-320011
VL  - 17
ER  -