TY  - JOUR
A1  - Heiser, A.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - Isolation of Islets of Langerhans: Improvement of the Isolation Technique Using the Pig Model.
N2  - During the last view years, interest in pancreatic islet transplantation for the cure of type I diabetes has increased markedly. A serious barrier to clinical islet transplantation is the isolation of a sufficient mass of viable and functional islets. We used a porcine islet isolation model to examine various parameters of the isolation procedure and to improve isolation technique.
KW  - Langerhans-Inseln
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87765
ER  - 
TY  - JOUR
A1  - Dickneite, G.
A1  - Schorlemmer, H. U.
A1  - Sedlacek, H. H.
A1  - Falk, W.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Suppression of macrophage function and prolongation of graft survival by the new guanidinic-like structure, 15-deoxyspergualin
N2  - No abstract available.
KW  - Makrophage
Y1  - 1987
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86991
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Korsgren, O.
A1  - Tibell, A.
A1  - Groth, C. G.
A1  - Müller-Ruchholtz, W.
T1  - Characterization of natural and induced human xenophile antibodies before and after transplantation of the fetal porcine endocrine pancreas
N2  - No abstract available.
KW  - Bauspeicheldrüse
KW  - Antikörper
KW  - Transplantation
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86985
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Heiser, A.
A1  - Müller-Ruchholtz, W.
T1  - Die zellvermittelte Immunantwort des menschlichen Transplantat Empfängers gegen xenogenes Antigen (Schwein) ist abhängig vom Spenderkompartiment.
N2  - No abstract available.
KW  - Transplantation
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86978
ER  - 
TY  - JOUR
A1  - Breitkreuz, A.
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Müller-Ruchholtz, W.
T1  - Induktion von Transplantat-Toleranz gegen Xenoantigen durch eine Pharmakon-Antigen-Kombinationsbehandlung
N2  - No abstract available.
KW  - Transplantat
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86969
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Gier, C.
A1  - Müller-Ruchholtz, W.
T1  - Isolierung Langerhans'scher Inseln aus dem Pankreas von Schweinen: Einfluss biologischer und technischer Parameter auf die Inselausbeite und -integrität
N2  - No abstract available.
KW  - Langerhans-Inseln
KW  - Isolierung <Mikrobiologie>
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-73223
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Eckstein, V.
A1  - Tibell, A.
A1  - Groth, C. G.
A1  - Korsgren, O.
A1  - Müller-Ruchholtz, W.
A1  - Thiede, A.
T1  - Natürliche und induzierte xenoreaktive Antikörper vor und nach klinischer Transplantation fötaler porziner Pankreasinselzellen
N2  - No abstract available.
KW  - Antikörper
KW  - Langerhans-Inseln
KW  - Schwein
KW  - Transplantation
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72967
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Heiser, A.
A1  - Bosse, M.
A1  - Wacker, H. H.
A1  - Müller-Ruchholtz, W.
T1  - Histologische Untersuchungen des Schweinepankreas zur Verbesserung der Inselausbeute und -integrität nach Kollagenase-Verdauung
N2  - No abstract available.
KW  - Schwein
KW  - Bauchspeicheldrüse
KW  - Histologie
KW  - Kollagenase
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-73237
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Pharmakologische Beeinflussung natürlicher Antikörper als Vorbedingung für die erfolgreiche Xenotransplantation
N2  - No abstract available.
KW  - Heterotransplantation
KW  - Antikörper
KW  - Pharmakologie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-73216
ER  - 
TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Winoto-Morbach, S.
A1  - Vanucchi, A.
A1  - Jäger, H.
A1  - Müller-Ruchholtz, W.
T1  - Erste Kieler Erfahrungen mit 45 Isolierungen von Langerhansinseln aus Schweinepankreas
N2  - No abstract available.
KW  - Langerhans-Inseln
KW  - Bauchspeicheldrüse
KW  - Schwein
Y1  - 1992
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-73205
ER  - 
TY  - JOUR
A1  - Winoto-Morbach, S.
A1  - Leyhausen, G.
A1  - Müller-Ruchholtz, W.
A1  - Ulrichs, Karin
T1  - The Electromagnetic Separation Principle: A Basis for Human Pancreatic Islet Transplantation
N2  - No abstract available
KW  - Allergie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-82520
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
T1  - Modulation of pancreatic islet allo immunogenicity and immunobiological in vitro and in vivo consequences
N2  - No abstract available.
KW  - Immunobiologie
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78261
ER  - 
TY  - JOUR
A1  - Laus, R.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Carbohydrate-specific human heterophile antibodies in normal human sera that react with xenogeneic cells
N2  - Human heterophile antibodies (HHA) that are present in normal human sera (NHS)play an important role in hyperacute xenograft rejection. The aim of this study was to analyze the occurrence, mode of action and molecular specificity of HHA in NHS that are directed against xenogeneic Iymphocytes (isolated from mouse, rat, guinea pig, rabbit, cattle and pig) and isolated rat pancreatic islets. All sera contained variable amounts of HHA that killed the target cells via the classical complement pathway. The cytotoxic activity of these HHA was specifically inhibited by certain carbohydrates (a-D-melibiose, ß-Iactose, ß-gentiobiose, ß-cellobiose, D-mannose, N-acetyl-ß-D-mannosamine and a-D-rhamnose) and by rat IgM. By means of affinity chromatography with immobilized inhibitors we obtained an antibody preparation of mainly IgG type from NHS (up to 3.5 mg/IO ml serum) that reacted strongly with rat lymphocytes and isolated rat pancreatic islets. Though thus far residual xenospecific antibody activity has remained in the sera even after multiple affinity chromatography, these data suggest that specific elimination of HHA is feasible and that it may be thus possible to overcome a major obstacle to xenotransplantation.
KW  - Immunobiologie
KW  - Chirurgie
Y1  - 1988
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-78271
ER  - 
TY  - JOUR
A1  - Krzymanski, M.
A1  - Waaga, A. M.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, Wolfgang
T1  - Long-standing rat kidney graft survival by a combination of organ perfusion with MHC class II monoclonal antibody and immunosuppression with reduced doses of 15-deoxyspergualin.
N2  - No abstract available
KW  - Niere
KW  - Ratte
KW  - MHC Klasse II
KW  - kidney
KW  - rat
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64442
ER  - 
TY  - JOUR
A1  - Strauss, Armin
A1  - Moskalenko, Vasily
A1  - Tiurbe, Christian
A1  - Chodnevskaja, Irina
A1  - Timm, Stephan
A1  - Wiegering, Verena A.
A1  - Germer, Chrioph Thomas
A1  - Ulrichs, Karin
T1  - Goettingen Minipigs (GMP): Comparison of Two Different Models for Inducing Diabetes
N2  - Purpose: Preclinical experiments on large animals are indispensable for evaluating the effectiveness of diabetes therapies. Miniature swine are well suited for such studies due to their physiological and pathophysiological responses. Methods: We compare two methods for inducing diabetes in Goettingen minipigs (GMP), in five with the beta cell toxin streptozotocin (STZ) and in five other GMP by total pancreatectomy (PE). Glucose homeostasis was assessed with the intravenous glucose-tolerance test (IVGTT) and continual monitoring of interstitial glucose levels. At conclusion of the observation period, the pancreata were examined histologically. Three non-diabetic GMP served as control group. Results: The IVGTT revealed markedly diabetic profiles in both GMP groups. STZ-GMP were found to harbor residual C-peptides and scattered insulin-positive cells in the pancreas. PE-GMP survived the total pancreatectomy only with intensive postoperative care. Conclusions: Although both methods reliably induced diabetes in GMP, the PE-GMP clearly had more health problems and required a greater expenditure of time and resources. The PE-GMP model, however, was better at eliminating endogenous insulin and C-peptide than the STZ-GMP model.
KW  - Göttingen
KW  - Minischwein
KW  - diabetes
KW  - pig or swine
KW  - real-time glucose monitoring
KW  - intravenous glucose tolerance test
KW  - total pancreatectomy
KW  - streptozotocin
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-75119
ER  - 
TY  - JOUR
A1  - Kekow, J.
A1  - Ulrichs, Karin
T1  - Determination of total immunoreactive rat insulin (IRI) in culture supernatants of rat islets by an enzyme linked immunosorbent assay (ELISA) as a routine method to assess the viability of rat islets prior to their use in islet transplantation
N2  - No abstract available
KW  - Immunobiologie
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72989
ER  - 
TY  - JOUR
A1  - Ulrichs, Karin
A1  - Keller, R.
A1  - Müller-Ruchholtz, W.
T1  - Serological demonstration and manipulation of passenger cells (PC) in pancreas islet grafts
N2  - No abstract available
KW  - Immunobiologie
Y1  - 1985
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72944
ER  - 
TY  - JOUR
A1  - Laus, R.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Molecular Specificity of Human Heterophile Antibodies (HHA) in Normal Human Serum (NHS) Reacting with Xenogeneic Cells.
N2  - No abstract available
KW  - Immunobiologie
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72921
ER  - 
TY  - JOUR
A1  - Krzymanski, Maciej
A1  - Waaga, Ana M.
A1  - Ulrichs, Karin
A1  - Deja, Aadam
A1  - Oko, Andrzej
A1  - Rommel, Thomas
A1  - Müller-Ruchholtz, Wolfgang
T1  - The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival
N2  - To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.
KW  - Chirurgie
KW  - Class II antigen blockade
KW  - rat
KW  - renal transplantation
KW  - Monoclonal antibody
KW  - dass II antigen blockade
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64431
ER  - 
TY  - JOUR
A1  - Deltz, Eberhard
A1  - Ulrichs, Karin
A1  - Schack, Thorsten
A1  - Friedrichs, Birgit
A1  - Müller-Ruchholtz, Wolfgang
A1  - Müller-Hermelink, Hans K.
A1  - Thiede, Arnulf
T1  - Graft-versus-host reaction in small bowel transplantation and possibilities for its circumvention
N2  - No abstract available
KW  - Dünndarm
KW  - Transplantation
KW  - transplantation
KW  - small bowel
Y1  - 1986
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-64425
ER  -