TY  - JOUR
A1  - Dimastrogiovanni, Daniela
A1  - Fröhlich, Kathrin S.
A1  - Bandyra, Katarzyna J.
A1  - Bruce, Heather A.
A1  - Hohensee, Susann
A1  - Vogel, Jörg
A1  - Luisi, Ben F.
T1  - Recognition of the small regulatory RNA RydC by the bacterial Hfq protein
JF  - eLife
N2  - Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at 3.48 angstrom resolution illuminates how the protein interacts with and presents the sRNA for target recognition. Consolidating the protein-RNA complex is a host of distributed interactions mediated by the natively unstructured termini of Hfq. Based on the structure and other data, we propose a model for a dynamic effector complex comprising Hfq, small RNA, and the cognate mRNA target.
KW  - Hfq
KW  - small RNA
KW  - natively unstructured protein
KW  - protein-RNA recognition
KW  - gene regulation
KW  - Escherichia coli-Hfq
KW  - SM-like protein
KW  - messenger-RNA
KW  - chaperone Hfq
KW  - target recognition
KW  - noncoding RNAs
KW  - interaction surfaces
KW  - crystal-structures
KW  - soluble-RNAs
KW  - C-Terminus
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-114191
SN  - 2050-084X
VL  - 3
IS  - e05375
ER  -