TY  - JOUR
A1  - Ban, Željka
A1  - Karačić, Zrinka
A1  - Tomić, Sanja
A1  - Amini, Hashem
A1  - Marder, Todd B.
A1  - Piantanida, Ivo
T1  - Triarylborane dyes as a novel non-covalent and non-inhibitive fluorimetric markers for DPP III enzyme
JF  - Molecules
N2  - Novel dyes were prepared by simple “click CuAAC” attachment of a triarylborane–alkyne to the azide side chain of an amino acid yielding triarylborane dye 1 which was conjugated with pyrene (dye 2) forming a triarylborane–pyrene FRET pair. In contrast to previous cationic triarylboranes, the novel neutral dyes interact only with proteins, while their affinity to DNA/RNA is completely abolished. Both the reference triarylborane amino acid and triarylborane–pyrene conjugate bind to BSA and the hDPP III enzyme with high affinities, exhibiting a strong (up to 100-fold) fluorescence increase, whereby the triarylborane–pyrene conjugate additionally retained FRET upon binding to the protein. Furthermore, the triarylborane dyes, upon binding to the hDPP III enzyme, did not impair its enzymatic activity under a wide range of experimental conditions, thus being the first non-covalent fluorimetric markers for hDPP III, also applicable during enzymatic reactions with hDPP III substrates.
KW  - triarylborane
KW  - pyrene
KW  - click CuAAC synthesis
KW  - DPP III enzyme
KW  - BSA
KW  - fluorescence
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-245046
SN  - 1420-3049
VL  - 26
IS  - 16
ER  -