TY  - JOUR
A1  - Herrmann, Marietta
A1  - Hildebrand, Maria
A1  - Menzel, Ursula
A1  - Fahy, Niamh
A1  - Alini, Mauro
A1  - Lang, Siegmund
A1  - Benneker, Lorin
A1  - Verrier, Sophie
A1  - Stoddart, Martin J.
A1  - Bara, Jennifer J.
T1  - Phenotypic characterization of bone marrow mononuclear cells and derived stromal cell populations from human iliac crest, vertebral body and femoral head
JF  - International Journal of Molecular Sciences
N2  - (1) In vitro, bone marrow-derived stromal cells (BMSCs) demonstrate inter-donor phenotypic variability, which presents challenges for the development of regenerative therapies. Here, we investigated whether the frequency of putative BMSC sub-populations within the freshly isolated mononuclear cell fraction of bone marrow is phenotypically predictive for the in vitro derived stromal cell culture. (2) Vertebral body, iliac crest, and femoral head bone marrow were acquired from 33 patients (10 female and 23 male, age range 14–91). BMSC sub-populations were identified within freshly isolated mononuclear cell fractions based on cell-surface marker profiles. Stromal cells were expanded in monolayer on tissue culture plastic. Phenotypic assessment of in vitro derived cell cultures was performed by examining growth kinetics, chondrogenic, osteogenic, and adipogenic differentiation. (3) Gender, donor age, and anatomical site were neither predictive for the total yield nor the population doubling time of in vitro derived BMSC cultures. The abundance of freshly isolated progenitor sub-populations (CD45−CD34−CD73+, CD45−CD34−CD146+, NG2+CD146+) was not phenotypically predictive of derived stromal cell cultures in terms of growth kinetics nor plasticity. BMSCs derived from iliac crest and vertebral body bone marrow were more responsive to chondrogenic induction, forming superior cartilaginous tissue in vitro, compared to those isolated from femoral head. (4) The identification of discrete progenitor populations in bone marrow by current cell-surface marker profiling is not predictive for subsequently derived in vitro BMSC cultures. Overall, the iliac crest and the vertebral body offer a more reliable tissue source of stromal progenitor cells for cartilage repair strategies compared to femoral head.
KW  - bone marrow stromal cells
KW  - MSC
KW  - pericytes
KW  - femoral head
KW  - vertebral body
KW  - iliac crest
KW  - chondrogenesis
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-285054
SN  - 1422-0067
VL  - 20
IS  - 14
ER  - 
TY  - JOUR
A1  - Herrmann, Marietta
A1  - Diederichs, Solvig
A1  - Melnik, Svitlana
A1  - Riegger, Jana
A1  - Trivanović, Drenka
A1  - Li, Shushan
A1  - Jenei-Lanzl, Zsuzsa
A1  - Brenner, Rolf E.
A1  - Huber-Lang, Markus
A1  - Zaucke, Frank
A1  - Schildberg, Frank A.
A1  - Grässel, Susanne
T1  - Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration
JF  - Frontiers in Bioengineering and Biotechnology
N2  - The incidence of musculoskeletal diseases is steadily increasing with aging of the population. In the past years, extracellular vesicles (EVs) have gained attention in musculoskeletal research. EVs have been associated with various musculoskeletal pathologies as well as suggested as treatment option. EVs play a pivotal role in communication between cells and their environment. Thereby, the EV cargo is highly dependent on their cellular origin. In this review, we summarize putative mechanisms by which EVs can contribute to musculoskeletal tissue homeostasis, regeneration and disease, in particular matrix remodeling and mineralization, pro-angiogenic effects and immunomodulatory activities. Mesenchymal stromal cells (MSCs) present the most frequently used cell source for EV generation for musculoskeletal applications, and herein we discuss how the MSC phenotype can influence the cargo and thus the regenerative potential of EVs. Induced pluripotent stem cell-derived mesenchymal progenitor cells (iMPs) may overcome current limitations of MSCs, and iMP-derived EVs are discussed as an alternative strategy. In the last part of the article, we focus on therapeutic applications of EVs and discuss both practical considerations for EV production and the current state of EV-based therapies.
KW  - extracellular vesicles
KW  - exosomes
KW  - musculoskeletal diseases
KW  - MSC
KW  - iMP
KW  - cell-free therapeutics
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-222882
SN  - 2296-4185
VL  - 8
ER  -