TY  - JOUR
A1  - Schmid, Rafael
A1  - Schmidt, Sonja K.
A1  - Hazur, Jonas
A1  - Detsch, Rainer
A1  - Maurer, Evelyn
A1  - Boccaccini, Aldo R.
A1  - Hauptstein, Julia
A1  - Teßmar, Jörg
A1  - Blunk, Torsten
A1  - Schrüfer, Stefan
A1  - Schubert, Dirk W.
A1  - Horch, Raymund E.
A1  - Bosserhoff, Anja K.
A1  - Arkudas, Andreas
A1  - Kengelbach-Weigand, Annika
T1  - Comparison of hydrogels for the development of well-defined 3D cancer models of breast cancer and melanoma
JF  - Cancers
N2  - Bioprinting offers the opportunity to fabricate precise 3D tumor models to study tumor pathophysiology and progression. However, the choice of the bioink used is important. In this study, cell behavior was studied in three mechanically and biologically different hydrogels (alginate, alginate dialdehyde crosslinked with gelatin (ADA–GEL), and thiol-modified hyaluronan (HA-SH crosslinked with PEGDA)) with cells from breast cancer (MDA-MB-231 and MCF-7) and melanoma (Mel Im and MV3), by analyzing survival, growth, and the amount of metabolically active, living cells via WST-8 labeling. Material characteristics were analyzed by dynamic mechanical analysis. Cell lines revealed significantly increased cell numbers in low-percentage alginate and HA-SH from day 1 to 14, while only Mel Im also revealed an increase in ADA–GEL. MCF-7 showed a preference for 1% alginate. Melanoma cells tended to proliferate better in ADA–GEL and HA-SH than mammary carcinoma cells. In 1% alginate, breast cancer cells showed equally good proliferation compared to melanoma cell lines. A smaller area was colonized in high-percentage alginate-based hydrogels. Moreover, 3% alginate was the stiffest material, and 2.5% ADA–GEL was the softest material. The other hydrogels were in the same range in between. Therefore, cellular responses were not only stiffness-dependent. With 1% alginate and HA-SH, we identified matrices that enable proliferation of all tested tumor cell lines while maintaining expected tumor heterogeneity. By adapting hydrogels, differences could be accentuated. This opens up the possibility of understanding and analyzing tumor heterogeneity by biofabrication.
KW  - breast cancer
KW  - melanoma
KW  - biofabrication
KW  - hydrogel
KW  - tumor heterogeneity
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-211195
SN  - 2072-6694
VL  - 12
IS  - 8
ER  - 
TY  - JOUR
A1  - Karakaya, Emine
A1  - Bider, Faina
A1  - Frank, Andreas
A1  - Teßmar, Jörg
A1  - Schöbel, Lisa
A1  - Forster, Leonard
A1  - Schrüfer, Stefan
A1  - Schmidt, Hans-Werner
A1  - Schubert, Dirk Wolfram
A1  - Blaeser, Andreas
A1  - Boccaccini, Aldo R.
A1  - Detsch, Rainer
T1  - Targeted printing of cells: evaluation of ADA-PEG bioinks for drop on demand approaches
JF  - Gels
N2  - A novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of cells onto a gel substrate with high cell survival rates in advanced bioinks. For this purpose, different alginate-dialdehyde—polyethylene glycol (ADA-PEG) inks with different PEG modifications and chain lengths (1–8 kDa) were characterized to evaluate their application as bioinks for drop on demand (DoD) printing. The biochemical properties of the inks, printing process, NIH/3T3 fibroblast cell distribution within a droplet and shear forces during printing were analyzed. Finally, different hydrogels were evaluated as a printing substrate. By analysing different PEG chain lengths with covalently crosslinked and non-crosslinked ADA-PEG inks, it was shown that the influence of Schiff's bases on the viscosity of the corresponding materials is very low. Furthermore, it was shown that longer polymer chains resulted in less stable hydrogels, leading to fast degradation rates. Several bioinks highly exhibit biocompatibility, while the calculated nozzle shear stress increased from approx. 1.3 and 2.3 kPa. Moreover, we determined the number of cells for printed droplets depending on the initial cell concentration, which is crucially needed for targeted cell printing approaches.
KW  - bioprinting
KW  - drop on demand
KW  - sodium alginate
KW  - polyethylene glycol
KW  - shear stress
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-267317
SN  - 2310-2861
VL  - 8
IS  - 4
ER  -