TY  - JOUR
A1  - Vargas Casanova, Yerly
A1  - Rodríguez Guerra, Jorge Antonio
A1  - Umaña Pérez, Yadi Adriana
A1  - Leal Castro, Aura Lucía
A1  - Almanzar Reina, Giovanni
A1  - García Castañeda, Javier Eduardo
A1  - Rivera Monroy, Zuly Jenny
T1  - Antibacterial synthetic peptides derived from bovine lactoferricin exhibit cytotoxic effect against MDA-MB-468 and MDA-MB-231 breast cancer cell lines
JF  - Molecules
N2  - Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B, containing the RRWQWR motif, were designed, synthesized, purified, and characterized using RP-HPLC chromatography and MALDI-TOF mass spectrometry. The antibacterial activity of the designed peptides against E. coli (ATCC 11775 and 25922) and their cytotoxic effect against MDA-MB-468 and MDA-MB-231 breast cancer cell lines were evaluated. Dimeric and tetrameric peptides showed higher antibacterial activity in both bacteria strains than linear peptides. The dimeric peptide (RRWQWR)\(_2\)K-Ahx exhibited the highest antibacterial activity against the tested bacterial strains. Furthermore, the peptides with high antibacterial activity exhibited significant cytotoxic effect against the tested breast cancer cell lines. This cytotoxic effect was fast and dependent on the peptide concentration. The tetrameric molecule containing RRWQWR motif has an optimal cytotoxic effect at a concentration of 22 µM. The evaluated dimeric and tetrameric peptides could be considered as candidates for developing new therapeutic agents against breast cancer. Polyvalence of linear sequences could be considered as a novel and versatile strategy for obtaining molecules with high anticancer activity.
KW  - lactoferricin B
KW  - E. coli
KW  - breast cancer
KW  - cytotoxic effect
KW  - antibacterial activity
KW  - synthetic peptides
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-173887
VL  - 22
IS  - 10
ER  -