TY  - JOUR
A1  - Neumann, Yvonne
A1  - Ohlsen, Knut
A1  - Donat, Stefanie
A1  - Engelmann, Susanne
A1  - Kusch, Harald
A1  - Albrecht, Dirk
A1  - Cartron, Michael
A1  - Hurd, Alexander
A1  - Foster, Simon J.
T1  - The effect of skin fatty acids on Staphylococcus aureus
JF  - Archives of Microbiology
N2  - Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS
KW  - S. aureus
KW  - skin fatty acid
KW  - C-6-H
KW  - resistance
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-121657
VL  - 197
ER  - 
TY  - JOUR
A1  - Selle, Martina
A1  - Hertlein, Tobias
A1  - Oesterreich, Babett
A1  - Klemm, Theresa
A1  - Kloppot, Peggy
A1  - Müller, Elke
A1  - Ehricht, Ralf
A1  - Stentzel, Sebastian
A1  - Bröker, Barbara M.
A1  - Engelmann, Susanne
A1  - Ohlsen, Knut
T1  - Global antibody response to Staphylococcus aureus live-cell vaccination
JF  - Scientific Reports
N2  - The pathogen Staphylococcus aureus causes a broad range of severe diseases and is feared for its ability to rapidly develop resistance to antibiotic substances. The increasing number of highly resistant S. aureus infections has accelerated the search for alternative treatment options to close the widening gap in anti-S. aureus therapy. This study analyses the humoral immune response to vaccination of Balb/c mice with sublethal doses of live S. aureus. The elicited antibody pattern in the sera of intravenously and intramuscularly vaccinated mice was determined using of a recently developed protein array. We observed a specific antibody response against a broad set of S. aureus antigens which was stronger following i.v. than i.m. vaccination. Intravenous but not intramuscular vaccination protected mice against an intramuscular challenge infection with a high bacterial dose. Vaccine protection was correlated with the strength of the anti-S. aureus antibody response. This study identified novel vaccine candidates by using protein microarrays as an effective tool and showed that successful vaccination against S. aureus relies on the optimal route of administration.
KW  - pathogens
KW  - bacterial infection
KW  - cell vaccines
KW  - Staphylococcus aureus
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-181245
VL  - 6
ER  -