TY  - JOUR
A1  - Schlagenhauf, Ulrich
A1  - Jakob, Lena
A1  - Eigenthaler, Martin
A1  - Segerer, Sabine
A1  - Jockel-Schneider, Yvonne
A1  - Rehn, Monika
T1  - Regular consumption of Lactobacillus reuteri-containing lozenges reduces pregnancy gingivitis: an RCT
JF  - Journal of Clinical Periodontology
N2  - Aim: 
This randomized controlled trial assessed the impact of Lactobacillus reuteri on pregnancy gingivitis in healthy women. 

Materials and Methods: 
Forty-five healthy women (24 test/21 placebo) with pregnancy gingivitis in the third trimester of pregnancy were enrolled. At baseline Gingival Index (GI) and Plaque Index (PlI) were assessed at the Ramfjord teeth and venous blood taken for TNF-alpha analysis. Subsequently participants were randomly provided with lozenges to be consumed 2 9 daily until birth (approx. 7 weeks) containing >= 10(8) CFU L. reuteri ATCC PTA 5289 and >= 10(8) CFU L. reuteri DSM 17938 (test) or being devoid of L. reuteri (placebo). Within 2 days after birth recording of GI, PlI and blood sampling were repeated. 

Results: 
At baseline, mean GI and mean PlI did not differ significantly between both groups. In the test group mean TNF-alpha serum level was significantly (p < 0.02) lower than in the placebo group. At reevaluation, mean GI and mean PlI of the test group were both significantly (p < 0.0001) lower than in the placebo group. Mean TNF-alpha serum level did no longer differ significantly between the groups. 

Conclusions: 
The consumption of L. reuteri lozenges may be a useful adjunct in the control of pregnancy gingivitis.
KW  - chronic periodontitis
KW  - probiotic lozenges
KW  - subgingival
KW  - bacteria
KW  - postpartum
KW  - microbiota
KW  - disease
KW  - L. reuteri
KW  - plaque
KW  - pregnancy
KW  - pregnancy gingivitis
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-186783
VL  - 43
IS  - 11
ER  - 
TY  - JOUR
A1  - Tinajero-Trejo, Mariana
A1  - Rana, Namrata
A1  - Nagel, Christoph
A1  - Jesse, Helen E.
A1  - Smith, Thomas W.
A1  - Wareham, Lauren K.
A1  - Hippler, Michael
A1  - Schatzschneider, Ulrich
A1  - Poole, Robert K.
T1  - Antimicrobial Activity of the Manganese Photoactivated Carbon Monoxide-Releasing Molecule [Mn(CO)\(_3\)(tpa-kappa\(^3\)N)]\(^+\) Against a Pathogenic Escherichia coli that Causes Urinary Infections
JF  - Antioxidants & Redox Signaling
N2  - Aims: We set out to investigate the antibacterial activity of a new Mn-based photoactivated carbon monoxide-releasing molecule (PhotoCORM, [Mn(CO)\(_3\)(tpa-kappa\(^3\)N)]\(^+\)) against an antibiotic-resistant uropathogenic strain (EC958) of Escherichia coli. Results: Activated PhotoCORM inhibits growth and decreases viability of E. coli EC958, but non-illuminated carbon monoxide-releasing molecule (CORM) is without effect. NADH-supported respiration rates are significantly decreased by activated PhotoCORM, mimicking the effect of dissolved CO gas. CO from the PhotoCORM binds to intracellular targets, namely respiratory oxidases in strain EC958 and a bacterial globin heterologously expressed in strain K-12. However, unlike previously characterized CORMs, the PhotoCORM is not significantly accumulated in cells, as deduced from the cellular manganese content. Activated PhotoCORM reacts avidly with hydrogen peroxide producing hydroxyl radicals; the observed peroxide-enhanced toxicity of the PhotoCORM is ameliorated by thiourea. The PhotoCORM also potentiates the effect of the antibiotic, doxycycline. Innovation: The present work investigates for the first time the antimicrobial activity of a light-activated PhotoCORM against an antibiotic-resistant pathogen. A comprehensive study of the effects of the PhotoCORM and its derivative molecules upon illumination is performed and mechanisms of toxicity of the activated PhotoCORM are investigated. Conclusion: The PhotoCORM allows a site-specific and time-controlled release of CO in bacterial cultures and has the potential to provide much needed information on the generality of CORM activities in biology. Understanding the mechanism(s) of activated PhotoCORM toxicity will be key in exploring the potential of this and similar compounds as antimicrobial agents, perhaps in combinatorial therapies with other agents.
KW  - intracellular hydrogen-peroxide
KW  - campylobacter-jejuni
KW  - oxygen-metabolism
KW  - deficient mutant
KW  - oxidative stress
KW  - aqueous-solution
KW  - metal caponyls
KW  - RU(CO)(3)CL(GLYCINATE)
KW  - bacteria
KW  - enzyme
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-188910
VL  - 24
IS  - 14
ER  -