TY - JOUR A1 - Ulrichs, Karin A1 - Eckstein, V. A1 - Müller-Buchholtz, W. T1 - Xenogeneic T-cell-mediated immune reactivity in the model of pig-to-humans: first findings with native stimulator cells N2 - No abstract available KW - Chirurgie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44755 ER - TY - JOUR A1 - Eckstein, V. A1 - Müller-Buchholtz, W. A1 - Ulrichs, Karin T1 - Reaction patterns of natural xenophile antibodies in human sera with pancreatic islet cells and porcine lymphocytes N2 - No abstract available KW - Chirurgie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44742 ER - TY - JOUR A1 - Heiser, A. A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, W. T1 - Prophylactic trypsin inhibition during the isolation procedure guarantees reproducible, high porcine islet yields N2 - Abstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields. KW - islets of Langerhans KW - xenogenic transplantation KW - swine KW - enzyme activation KW - enzyme inhibitors Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45531 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Eckstein, V. A1 - Tibell, A. A1 - Groth, C. G. A1 - Korsgren, O. A1 - Müller-Ruchholtz, W. A1 - Thiede, A. T1 - Natürliche und induzierte xenoreaktive Antikörper vor und nach klinischer Transplantation fötaler porziner Pankreasinselzellen N2 - No abstract available. KW - Antikörper KW - Langerhans-Inseln KW - Schwein KW - Transplantation Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-72967 ER - TY - JOUR A1 - Winoto-Morbach, S. A1 - Krout, OS A1 - Heiser, A. A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, W. T1 - Lectin binding to acinar tissue for complete magnetophoretic purification of porcine pancreatic islets depends on the composition and pH of the incubation medium N2 - No abstract available KW - Chirurgie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45183 ER - TY - JOUR A1 - Heiser, Axel A1 - Ulrichs, Karin A1 - Müller-Buchholtz, Wolfgang T1 - Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields N2 - During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has Increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardized and collagenase of controlled quality was used, we were unable to attain high islet yields with a satisfactory degree of reproducibility. One hypothesis was that varying degrees of interference by donor pancreatic enzymes were responsible for this failure. The aim of this stUdy was to examine the kinetics of four types of enzymatic activity during the isolation procedure, as well as their effects on islet yield: collagenase, trypsin, neutral protease, and clostripaln. Our results indicate that while exogenous collagenase activity decreases slightly during the isolation procedure, the activity of the pancreas enzymes neutral protease and trypsin increases. In some cases, trypsin activity increases very strongly. A strong increase in trypsin activity correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Addition of the protease inhibitor Pefabloc to the isolation medium results in low trypsin activity and reproducible high islet yields. KW - Langerhans-Inseln KW - islets of Langerhans KW - xenogeneic transplantation KW - swine KW - enzyme activation KW - enzyme inhibitors Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44719 ER - TY - JOUR A1 - Heiser, A. A1 - Müller-Ruchholtz, W. A1 - Ulrichs, Karin T1 - Isolation of Islets of Langerhans: Improvement of the Isolation Technique Using the Pig Model. N2 - During the last view years, interest in pancreatic islet transplantation for the cure of type I diabetes has increased markedly. A serious barrier to clinical islet transplantation is the isolation of a sufficient mass of viable and functional islets. We used a porcine islet isolation model to examine various parameters of the isolation procedure and to improve isolation technique. KW - Langerhans-Inseln Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-87765 ER - TY - JOUR A1 - Heiser, A. A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, W. T1 - Influence of porcine strain, age, and pH of the isolation medium on porcine pancreatic islet isolation success N2 - No abstract available KW - Chirurgie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45171 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Bosse, M. A1 - Wacker, HH A1 - Heiser, A. A1 - Müller-Ruchholtz, W. T1 - Histologic analysis of the porcine pancreas to improve islet yield and integrity after collagenase digestion N2 - No abstract available KW - Chirurgie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45166 ER - TY - CHAP A1 - Heiser, A. A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, W. T1 - Enzyme Kinetics of Commercial Collagenases and their Influence on Porcine Islet Isolation N2 - No abstract available KW - Heterotransplantation Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45488 ER - TY - CHAP A1 - Heiser, A. A1 - Ulrichs, Karin A1 - Müller-Ruchholtz, W. T1 - Enzyme Activities in Commercial Collagenase Preparations and Their Kinetics During Islet Isolation Procedure N2 - No abstract available KW - Transplantation Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-45633 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Wang, H. A1 - Müller-Buchholtz, W. T1 - Down-regulation of xenophile antibodies by 15-deoxyspergualin in an experimental animal model N2 - No abstract available KW - Chirurgie Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-44733 ER - TY - JOUR A1 - Ulrichs, Karin A1 - Eckstein, V. A1 - Korsgren, O. A1 - Tibell, A. A1 - Groth, C. G. A1 - Müller-Ruchholtz, W. T1 - Characterization of natural and induced human xenophile antibodies before and after transplantation of the fetal porcine endocrine pancreas N2 - No abstract available. KW - Bauspeicheldrüse KW - Antikörper KW - Transplantation Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-86985 ER -