TY  - JOUR
A1  - Meier, Doreen
A1  - Kruse, Janis
A1  - Buttlar, Jann
A1  - Friedrich, Michael
A1  - Zenk, Fides
A1  - Boesler, Benjamin
A1  - Forstner, Konrad U.
A1  - Hammann, Christian
A1  - Nellen, Wolfgang
T1  - Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein
JF  - PLoS Genetics
N2  - We identified the dsRNA binding protein RbdB as an essential component in miRNA processing in Dictyostelium discoideum. RbdB is a nuclear protein that accumulates, together with Dicer B, in nucleolar foci reminiscent of plant dicing bodies. Disruption of rbdB results in loss of miRNAs and accumulation of primary miRNAs. The phenotype can be rescued by ectopic expression of RbdB thus allowing for a detailed analysis of domain function. The lack of cytoplasmic dsRBD proteins involved in miRNA processing, suggests that both processing steps take place in the nucleus thus resembling the plant pathway. However, we also find features e.g. in the domain structure of Dicer which suggest similarities to animals. Reduction of miRNAs in the rbdB- strain and their increase in the Argonaute A knock out allowed the definition of new miRNAs one of which appears to belong to a new non-canonical class.
KW  - microprocessor
KW  - Dictyostelium discoideum
KW  - dsRNA binding protein
KW  - RbdB
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166687
VL  - 12
IS  - 6
ER  - 
TY  - JOUR
A1  - Esser, Peter
A1  - Mehnert‐Theuerkauf, Anja
A1  - Friedrich, Michael
A1  - Johansen, Christoffer
A1  - Brähler, Elmar
A1  - Faller, Hermann
A1  - Härter, Martin
A1  - Koch, Uwe
A1  - Schulz, Holger
A1  - Wegscheider, Karl
A1  - Weis, Joachim
A1  - Kuba, Katharina
A1  - Hinz, Andreas
A1  - Hartung, Tim
T1  - Risk and associated factors of depression and anxiety in men with prostate cancer: Results from a German multicenter study
JF  - Psycho‐Oncology
N2  - Objective
In order to optimize psycho‐oncological care, studies that quantify the extent of distress and identify certain risk groups are needed. Among patients with prostate cancer (PCa), findings on depression and anxiety are limited.

Methods
We analyzed data of PCa patients selected from a German multi‐center study. Depression and anxiety were assessed with the PHQ‐9 and the GAD‐7 (cut‐off ≥7). We provided physical symptom burden, calculated absolute and relative risk (AR and RR) of depression and anxiety across patient subsets and between patients and the general population (GP) and tested age as a moderator within the relationship of disease‐specific symptoms with depression and anxiety.

Results
Among 636 participants, the majority reported disease‐specific problems (sexuality: 60%; urination: 52%). AR for depression and anxiety was 23% and 22%, respectively. Significant RR were small, with higher risks of distress in patients who are younger (eg, RR\(_{depression}\) = 1.15; 95%‐CI: 1.06‐1.26), treated with chemotherapy (RR\(_{depression}\)n = 1.46; 95%‐CI: 1.09‐1.96) or having metastases (RR\(_{depression}\) = 1.30; 95%‐CI: 1.02‐1.65). Risk of distress was slightly elevated compared to GP (eg, RR\(_{depression}\) = 1.13; 95%‐CI: 1.07‐1.19). Age moderated the relationship between symptoms and anxiety (B\(_{urination}\) = −0.10, P = .02; B\(_{sexuality}\) = −0.11, P = .01).

Conclusions
Younger patients, those with metastases or treatment with chemotherapy seem to be at elevated risk for distress and should be closely monitored. Many patients suffer from disease‐specific symptom burden, by which younger patients seem to be particularly distressed. Support of coping mechanisms associated with disease‐specific symptom burden seems warranted.
KW  - anxiety
KW  - cancer
KW  - depression
KW  - oncology
KW  - prostatic neoplasms
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-218277
VL  - 29
IS  - 10
SP  - 1604
EP  - 1612
ER  -