TY  - JOUR
A1  - Gordon, Sarah
A1  - Daneshian, Mardas
A1  - Bouwstra, Joke
A1  - Caloni, Francesca
A1  - Constant, Samuel
A1  - Davies, Donna E.
A1  - Dandekar, Gudrun
A1  - Guzman, Carlos A.
A1  - Fabian, Eric
A1  - Haltner, Eleonore
A1  - Hartung, Thomas
A1  - Hasiwa, Nina
A1  - Hayden, Patrick
A1  - Kandarova, Helena
A1  - Khare, Sangeeta
A1  - Krug, Harald F.
A1  - Kneuer, Carsten
A1  - Leist, Marcel
A1  - Lian, Guoping
A1  - Marx, Uwe
A1  - Metzger, Marco
A1  - Ott, Katharina
A1  - Prieto, Pilar
A1  - Roberts, Michael S.
A1  - Roggen, Erwin L.
A1  - Tralau, Tewes
A1  - van den Braak, Claudia
A1  - Walles, Heike
A1  - Lehr, Claus-Michael
T1  - Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology
JF  - ALTEX: Alternatives to Animal Experimentation
N2  - Models of the outer epithelia of the human body namely the skin, the intestine and the lung have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.
KW  - on-a-chip
KW  - asthmatic bronchial epithelium
KW  - vesicle-based barrier
KW  - pulmonary drug-delivery
KW  - epithelial cell culture
KW  - cytotoxicity
KW  - transport studies
KW  - permeability
KW  - in vitro models
KW  - air-liquid interface
KW  - respiratory syncytial virus
KW  - reconstructed human epidermis
KW  - artificial membrane-permeability
KW  - embryonic stem cells
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-144275
VL  - 32
IS  - 4
ER  - 
TY  - JOUR
A1  - Pereira, A. R.
A1  - Trivanović, D.
A1  - Herrmann, M.
T1  - Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro
JF  - European Cells and Materials
N2  - Mesenchymal stem/stromal cells (MSCs) are an essential element of most modern tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease – including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.
KW  - Mesenchymal stem/stromal cells
KW  - skeletal progenitor cells
KW  - niche
KW  - in vitro models
KW  - bone
KW  - tissue engineering
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-268823
SN  - 1473-2262
VL  - 37
ER  -