TY  - JOUR
A1  - Strobel, Lea
A1  - Johswich, Kay O.
T1  - Anticoagulants impact on innate immune responses and bacterial survival in whole blood models of Neisseria meningitidis infection
JF  - Scientific Reports
N2  - Neisseria meningitidis (meningococcus) causes invasive diseases such as meningitis or septicaemia. Ex vivo infection of human whole blood is a valuable tool to study meningococcal virulence factors and the host innate immune responses. In order to consider effects of cellular mediators, the coagulation cascade must be inhibited to avoid clotting. There is considerable variation in the anticoagulants used among studies of N. meningitidis whole blood infections, featuring citrate, heparin or derivatives of hirudin, a polypeptide from leech saliva. Here, we compare the influence of these three different anticoagulants, and additionally Mg/EGTA, on host innate immune responses as well as on viability of N. meningitidis strains isolated from healthy carriers and disease cases, reflecting different sequence types and capsule phenotypes. We found that the anticoagulants significantly impact on cellular responses and, strain-dependently, also on bacterial survival. Hirudin does not inhibit complement and is therefore superior over the other anticoagulants; indeed hirudin-plasma most closely reflects the characteristics of serum during N. meningitidis infection. We further demonstrate the impact of heparin on complement activation on N. meningitidis and its consequences on meningococcal survival in immune sera, which appears to be independent of the heparin binding antigens Opc and NHBA.
KW  - infection
KW  - pathogens
KW  - Neisseria meningitidis
KW  - anticoagulants
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-176226
VL  - 8
IS  - 10225
ER  - 
TY  - JOUR
A1  - Albers, Gregory W.
A1  - Bernstein, Richard A.
A1  - Brachmann, Johannes
A1  - Camm, John
A1  - Easton, J. Donald
A1  - Fromm, Peter
A1  - Goto, Shinya
A1  - Granger, Christopher B.
A1  - Hohnloser, Stefan H.
A1  - Hylek, Elaine
A1  - Jaffer, Amir K.
A1  - Krieger, Derk W.
A1  - Passman, Rod
A1  - Pines, Jesse M.
A1  - Reed, Shelby D.
A1  - Rothwell, Peter M.
A1  - Kowey, Peter R.
T1  - Heart Rhythm Monitoring Strategies for Cryptogenic Stroke: 2015 Diagnostics and Monitoring Stroke Focus Group Report
JF  - Journal of the American Heart Association
N2  - No abstract available.
KW  - anticoagulants
KW  - atrial fibrillation
KW  - diagnosis
KW  - electrocardiography
KW  - insertable cardiac monitor
KW  - stroke prevention
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-165709
VL  - 5
IS  - e00294
ER  - 
TY  - JOUR
A1  - Fluri, Felix
A1  - Heinen, Florian
A1  - Kleinschnitz, Christoph
T1  - Intravenous Thrombolysis in a Stroke Patient Receiving Rivaroxaban
JF  - Cerebrovascular Disease Extra
N2  - No abstract available.
KW  - anticoagulants
KW  - intravenous thrombolysis
KW  - acute ischemic stroke
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-128816
VL  - 2013
IS  - 3
ER  -