TY  - JOUR
A1  - Chen, Jiangtian
A1  - Reiher, Wencke
A1  - Hermann-Luibl, Christiane
A1  - Sellami, Azza
A1  - Cognigni, Paola
A1  - Kondo, Shu
A1  - Helfrich-Förster, Charlotte
A1  - Veenstra, Jan A.
A1  - Wegener, Christian
T1  - Allatostatin A Signalling in Drosophila Regulates Feeding and Sleep and Is Modulated by PDF
JF  - PLoS Genetics
N2  - Feeding and sleep are fundamental behaviours with significant interconnections and cross-modulations. The circadian system and peptidergic signals are important components of this modulation, but still little is known about the mechanisms and networks by which they interact to regulate feeding and sleep. We show that specific thermogenetic activation of peptidergic Allatostatin A (AstA)-expressing PLP neurons and enteroendocrine cells reduces feeding and promotes sleep in the fruit fly Drosophila. The effects of AstA cell activation are mediated by AstA peptides with receptors homolog to galanin receptors subserving similar and apparently conserved functions in vertebrates. We further identify the PLP neurons as a downstream target of the neuropeptide pigment-dispersing factor (PDF), an output factor of the circadian clock. PLP neurons are contacted by PDF-expressing clock neurons, and express a functional PDF receptor demonstrated by cAMP imaging. Silencing of AstA signalling and continuous input to AstA cells by tethered PDF changes the sleep/activity ratio in opposite directions but does not affect rhythmicity. Taken together, our results suggest that pleiotropic AstA signalling by a distinct neuronal and enteroendocrine AstA cell subset adapts the fly to a digestive energy-saving state which can be modulated by PDF.
KW  - neurons
KW  - neuroimaging
KW  - circadian rhythms
KW  - food consumption
KW  - sleep
KW  - biological locomotion
KW  - Drosophila melanogaster
KW  - signal peptides
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-178170
VL  - 12
IS  - 9
ER  - 
TY  - JOUR
A1  - Buchner, Erich
A1  - Blanco Redondo, Beatriz
A1  - Bunz, Melanie
A1  - Halder, Partho
A1  - Sadanandappa, Madhumala K.
A1  - Mühlbauer, Barbara
A1  - Erwin, Felix
A1  - Hofbauer, Alois
A1  - Rodrigues, Veronica
A1  - VijayRaghavan, K.
A1  - Ramaswami, Mani
A1  - Rieger, Dirk
A1  - Wegener, Christian
A1  - Förster, Charlotte
T1  - Identification and Structural Characterization of Interneurons of the Drosophila Brain by Monoclonal Antibodies of the Würzburg Hybridoma Library
JF  - PLoS ONE
N2  - Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs) of the Würzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Würzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.
KW  - cell staining
KW  - drosophila melanogaster
KW  - gene expression
KW  - hybridomas
KW  - immune serum
KW  - library screening
KW  - monoclonal antibodies
KW  - neurons
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-97109
ER  -