TY  - JOUR
A1  - Liu, Yi
A1  - Maierhofer, Tobias
A1  - Rybak, Katarzyna
A1  - Sklenar, Jan
A1  - Breakspear, Andy
A1  - Johnston, Matthew G.
A1  - Fliegmann, Judith
A1  - Huang, Shouguang
A1  - Roelfsema, M. Rob G.
A1  - Felix, Georg
A1  - Faulkner, Christine
A1  - Menke, Frank L.H.
A1  - Geiger, Dietmar
A1  - Hedrich, Rainer
A1  - Robatzek, Silke
T1  - Anion channel SLAH3 is a regulatory target of chitin receptor-associated kinase PBL27 in microbial stomatal closure
JF  - eLife
N2  - In plants, antimicrobial immune responses involve the cellular release of anions and are responsible for the closure of stomatal pores. Detection of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) induces currents mediated via slow-type (S-type) anion channels by a yet not understood mechanism. Here, we show that stomatal closure to fungal chitin is conferred by the major PRRs for chitin recognition, LYK5 and CERK1, the receptor-like cytoplasmic kinase PBL27, and the SLAH3 anion channel. PBL27 has the capacity to phosphorylate SLAH3, of which S127 and S189 are required to activate SLAH3. Full activation of the channel entails CERK1, depending on PBL27. Importantly, both S127 and S189 residues of SLAH3 are required for chitin-induced stomatal closure and anti-fungal immunity at the whole leaf level. Our results demonstrate a short signal transduction module from MAMP recognition to anion channel activation, and independent of ABA-induced SLAH3 activation.
KW  - plants
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202631
VL  - 8
ER  - 
TY  - JOUR
A1  - Jaślan, Dawid
A1  - Dreyer, Ingo
A1  - Lu, Jinping
A1  - O'Malley, Ronan
A1  - Dindas, Julian
A1  - Marten, Irene
A1  - Hedrich, Rainer
T1  - Voltage-dependent gating of SV channel TPC1 confers vacuole excitability
JF  - Nature Communications
N2  - In contrast to the plasma membrane, the vacuole membrane has not yet been associated with electrical excitation of plants. Here, we show that mesophyll vacuoles from Arabidopsis sense and control the membrane potential essentially via the K\(^+\)-permeable TPC1 and TPK channels. Electrical stimuli elicit transient depolarization of the vacuole membrane that can last for seconds. Electrical excitability is suppressed by increased vacuolar Ca\(^{2+}\) levels. In comparison to wild type, vacuoles from the fou2 mutant, harboring TPC1 channels insensitive to luminal Ca\(^{2+}\), can be excited fully by even weak electrical stimuli. The TPC1-loss-of-function mutant tpc1-2 does not respond to electrical stimulation at all, and the loss of TPK1/TPK3-mediated K\(^{+}\) transport affects the duration of TPC1-dependent membrane depolarization. In combination with mathematical modeling, these results show that the vacuolar K\(^+\)-conducting TPC1 and TPK1/TPK3 channels act in concert to provide for Ca\(^{2+}\)- and voltage-induced electrical excitability to the central organelle of plant cells.
KW  - Biophysics
KW  - Plant signalling
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202029
VL  - 10
ER  -