TY  - JOUR
A1  - Kuznetsov, Nikolai V.
A1  - Almuzzaini, Bader
A1  - Kritikou, Joanna S.
A1  - Baptista, Marisa A. P.
A1  - Oliveira, Mariana M. S.
A1  - Keszei, Marton
A1  - Snapper, Scott B.
A1  - Percipalle, Piergiorgio
A1  - Westerberg, Lisa S.
T1  - Nuclear Wiskott-Aldrich syndrome protein co-regulates T cell factor 1-mediated transcription in T cells
JF  - Genome Medicine
N2  - Background
The Wiskott–Aldrich syndrome protein (WASp) family of actin-nucleating factors are present in the cytoplasm and in the nucleus. The role of nuclear WASp for T cell development remains incompletely defined.

Methods
We performed WASp chromatin immunoprecipitation and deep sequencing (ChIP-seq) in thymocytes and spleen CD4\(^+\) T cells.

Results
WASp was enriched at genic and intergenic regions and associated with the transcription start sites of protein-coding genes. Thymocytes and spleen CD4\(^+\) T cells showed 15 common WASp-interacting genes, including the gene encoding T cell factor (TCF)12. WASp KO thymocytes had reduced nuclear TCF12 whereas thymocytes expressing constitutively active WASp\(^{L272P}\) and WASp\(^{I296T}\) had increased nuclear TCF12, suggesting that regulated WASp activity controlled nuclear TCF12. We identify a putative DNA element enriched in WASp ChIP-seq samples identical to a TCF1-binding site and we show that WASp directly interacted with TCF1 in the nucleus.

Conclusions
These data place nuclear WASp in proximity with TCF1 and TCF12, essential factors for T cell development.
KW  - Medicine
KW  - WASp
KW  - T cells
KW  - ChIP-seq
KW  - Wiskott-Aldrich syndrome
KW  - TCF1
KW  - TCF12
KW  - Nucleus
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-173486
VL  - 9
ER  -