TY  - JOUR
A1  - Conrad, Thomas
A1  - Albrecht, Anne-Susann
A1  - Rodrigues de Melo Costa, Veronica
A1  - Sauer, Sascha
A1  - Meierhofer, David
A1  - Andersson Ørom, Ulf
T1  - Serial interactome capture of the human cell nucleus
JF  - Nature Communications
N2  - Novel RNA-guided cellular functions are paralleled by an increasing number of RNA-binding proteins (RBPs). Here we present ‘serial RNA interactome capture’ (serIC), a multiple purification procedure of ultraviolet-crosslinked poly(A)–RNA–protein complexes that enables global RBP detection with high specificity. We apply serIC to the nuclei of proliferating K562 cells to obtain the first human nuclear RNA interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from previous IC experiments, including few factors without known RNA-binding domains that are in good agreement with computationally predicted RNA binding. serIC extends the number of DNA–RNA-binding proteins (DRBPs), and reveals a network of RBPs involved in p53 signalling and double-strand break repair. serIC is an effective tool to couple global RBP capture with additional selection or labelling steps for specific detection of highly purified RBPs.
KW  - human cell nucleus
KW  - serial RNA interactome capture
KW  - RNA-binding proteins
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166172
VL  - 7
IS  - 11212
ER  -